Difference between revisions of "Part:BBa K2404001:Design"
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| − | - We ordered the following TSH gBlock from IDT | + | - We ordered the following TSH gBlock from IDT<br> |
| − | < | + | https://static.igem.org/mediawiki/parts/2/2d/T--Cardiff_Wales--TSH_gblock.png |
| − | - This was introduced into | + | - This was introduced into [https://parts.igem.org/Part:BBa_P10500 BBa_P10500] by digestion with BsmBI. This generated a 5' and 3' BsaI sites for level 1 cloning |
===Source=== | ===Source=== | ||
| − | This modified protein was | + | This modified protein was first used in [http://www.jbc.org/content/276/7/4543.long Fares et al (2001) JBC 276, 4543-4558] but without the His-tags. |
It was ordered as a g-block. | It was ordered as a g-block. | ||
===References=== | ===References=== | ||
Latest revision as of 21:00, 30 October 2017
A Thyroid Stimulating Hormone antagonist with His-Tags
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 540
Illegal PstI site found at 582 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 540
Illegal PstI site found at 582 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 795
Illegal XhoI site found at 607 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 540
Illegal PstI site found at 582 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 540
Illegal PstI site found at 582 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 827
Design Notes
We generated this part through the following steps:
- We ordered the following TSH gBlock from IDT
- This was introduced into BBa_P10500 by digestion with BsmBI. This generated a 5' and 3' BsaI sites for level 1 cloning
Source
This modified protein was first used in [http://www.jbc.org/content/276/7/4543.long Fares et al (2001) JBC 276, 4543-4558] but without the His-tags. It was ordered as a g-block.