Difference between revisions of "Part:BBa K2404002"
 
(5 intermediate revisions by 2 users not shown)
Line 3: Line 3:
 
<partinfo>BBa_K2404002 short</partinfo>
 
<partinfo>BBa_K2404002 short</partinfo>
  
−
This is the most 3’ 500bp of an inducible promoter that has been isolated from <i> Arabidopsis thaliana </i>. It responds to jasmonic acid. This part is suitable for Golden Gate cloning using type IIS restriction enzymes.
+
The [https://apps.araport.org/thalemine/portal.do?externalids=AT5G44420 PDF1.2 promotor] is a regulatory sequence from <i>Arabidopsis thaliana</i> that has been shown to respond to jasmonic acid.  
 +
 
 +
It is a truncated version of [https://parts.igem.org/Part:BBa_K1467103 BBa_K1467103]
 +
 
 +
This promotor includes 500bp upstream of the PDF1.2 translational start point.
 +
 
 +
This part conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard and contains the 5' GGAG and 3' AATG sequences for cloning into level 1 plasmids.
 +
 +
This part is has been cloned into the [https://parts.igem.org/Part:BBa_P10500 P10500] plasmid using BsmBI.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 10: Line 18:
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
−
<partinfo>BBa_K2404000 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K2404002 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
−
<partinfo>BBa_K2404000 parameters</partinfo>
+
<partinfo>BBa_K2404002 parameters</partinfo>
 
<!-- -->
 
<!-- -->

Latest revision as of 21:13, 30 October 2017


PDF1.2 shortened - a jasmonic acid-induced promoter

The PDF1.2 promotor is a regulatory sequence from Arabidopsis thaliana that has been shown to respond to jasmonic acid.

It is a truncated version of BBa_K1467103

This promotor includes 500bp upstream of the PDF1.2 translational start point.

This part conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard and contains the 5' GGAG and 3' AATG sequences for cloning into level 1 plasmids.

This part is has been cloned into the P10500 plasmid using BsmBI.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 408
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 517