Difference between revisions of "Part:BBa I739002"
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===Part Structure=== | ===Part Structure=== | ||
− | <p>The Biobrick encodes [https://parts.igem.org/wiki/index.php/Help:Tag LVA]-tagged | + | <p>The Biobrick encodes [https://parts.igem.org/wiki/index.php/Help:Tag LVA]-tagged LacI ([https://parts.igem.org/wiki/index.php/Part:BBa_C0012 BBa_C0012]) under control of the constitutive promoter [https://parts.igem.org/wiki/index.php/Part:BBa_J23105 BBa_J23105] followed by the ribosome binding site [https://parts.igem.org/wiki/index.php/Part:BBa_B0034 BBa_B0034]. The transcription of lacI is terminated by the double terminator [https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015].</p> |
===Mode of Action=== | ===Mode of Action=== | ||
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===Purpose=== | ===Purpose=== | ||
− | <p>This Biobrick was designed for the [http:// | + | <p>This Biobrick was designed for the [http://2007.igem.org/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. In the project description, this part is also termed ''Part 2''. The constitutively synthesized LacI interacts with the double promoters [https://parts.igem.org/wiki/index.php/Part:BBa_I739103 BBa_I739103] and [https://parts.igem.org/wiki/index.php/Part:BBa_I739107 BBa_I739107] which are parts of composites [https://parts.igem.org/wiki/index.php/Part:BBa_I739016 BBa_I739016] and [https://parts.igem.org/wiki/index.php/Part:BBa_I739011 BBa_I739011] respectively. A similar (but not used) construct in this context is [https://parts.igem.org/wiki/index.php/Part:BBa_Q04121 BBa_Q04121].</p> |
===Testing=== | ===Testing=== | ||
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Pst: ok<br> | Pst: ok<br> | ||
</p> | </p> | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 19:49, 26 October 2007
Constitutive expression cassette for LacI +LVA (J23100.B0034.C0012.B0015)
Part Structure
The Biobrick encodes LVA-tagged LacI (BBa_C0012) under control of the constitutive promoter BBa_J23105 followed by the ribosome binding site BBa_B0034. The transcription of lacI is terminated by the double terminator BBa_B0015.
Mode of Action
LacI binds to the pLac regulator (BBa_R0010) (or to the PLlac01 hybrid regulator BBa_R0011 respectively) and represses transcription. If the inducer [http://openwetware.org/wiki/IPTG Isopropyl-beta-D-thiogalactopyranoside (IPTG)] is added, lacI action is inhibited and the promoter gets derepressed.
Purpose
This Biobrick was designed for the [http://2007.igem.org/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. In the project description, this part is also termed Part 2. The constitutively synthesized LacI interacts with the double promoters BBa_I739103 and BBa_I739107 which are parts of composites BBa_I739016 and BBa_I739011 respectively. A similar (but not used) construct in this context is BBa_Q04121.
Testing
Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1208
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]