Difference between revisions of "Part:BBa M50013:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| + | arsR produces a repressor protein that binds to the arsR promoter. When arsR levels are sufficiently high, transcription is repressed until arsenic binds to these repressors and allows transcription to start. Additional protein-coding genes can be placed after this promoter-repressor unit. These proteins will only be expressed in the presence of arsenic. | ||
| + | This mechanism is originally from the ars operon in E. Coli. This operon consists of five genes: arsR, arsD, arsA, arsB, and arsC (Suzuki et al. 1998. Expression and Regulation of the Arsenic Resistance Operon of Acidiphilium multivorum AIU 301 Plasmid pKW301 in Escherichia coli. Appl Environ Microbiol. 64(2): 411–418). The first two encode repressor proteins that repress transcription of the entire set of genes. The last three encode proteins that transport arsenic out of the cell (Carlin, A., Shi, W., Rosen, B.P. 1995. The ars Operon of Escherichia coli Confers Arsenical and Antimonial Resistance. Journal of Bacteriology. 177(4): 981-986). These resistance proteins are only expressed in the presence of arsenic. This composite part consists only of the promoter region and the gene for the arsR protein (repressor). No changes were made. The sequences were used directly from the arsenic resistance plasmid found in E.coli. | ||
===Source=== | ===Source=== | ||
| − | This part can be found in E. Coli chromosomal and plasmid DNA. | + | This part can be found in E. Coli chromosomal and plasmid DNA. (Escherichia coli K-12 substr. MG1655 arsR. (2016). Ecocyc.org. Retrieved 26 October 2016, from http://ecocyc.org/gene?orgid=ECOLI&id=EG12235#tab=REGULON). |
===References=== | ===References=== | ||
Latest revision as of 02:46, 10 December 2016
Arsenic Sensor (arsR operon promoter and arsR gene)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 159
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
arsR produces a repressor protein that binds to the arsR promoter. When arsR levels are sufficiently high, transcription is repressed until arsenic binds to these repressors and allows transcription to start. Additional protein-coding genes can be placed after this promoter-repressor unit. These proteins will only be expressed in the presence of arsenic. This mechanism is originally from the ars operon in E. Coli. This operon consists of five genes: arsR, arsD, arsA, arsB, and arsC (Suzuki et al. 1998. Expression and Regulation of the Arsenic Resistance Operon of Acidiphilium multivorum AIU 301 Plasmid pKW301 in Escherichia coli. Appl Environ Microbiol. 64(2): 411–418). The first two encode repressor proteins that repress transcription of the entire set of genes. The last three encode proteins that transport arsenic out of the cell (Carlin, A., Shi, W., Rosen, B.P. 1995. The ars Operon of Escherichia coli Confers Arsenical and Antimonial Resistance. Journal of Bacteriology. 177(4): 981-986). These resistance proteins are only expressed in the presence of arsenic. This composite part consists only of the promoter region and the gene for the arsR protein (repressor). No changes were made. The sequences were used directly from the arsenic resistance plasmid found in E.coli.
Source
This part can be found in E. Coli chromosomal and plasmid DNA. (Escherichia coli K-12 substr. MG1655 arsR. (2016). Ecocyc.org. Retrieved 26 October 2016, from http://ecocyc.org/gene?orgid=ECOLI&id=EG12235#tab=REGULON).