Difference between revisions of "Part:BBa M50013"
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<partinfo>BBa_M50013 short</partinfo> | <partinfo>BBa_M50013 short</partinfo> | ||
| − | This part is a subset of the ars operon, which consists of a promoter and 5 genes: arsR, arsD, arsA, arsB, and arsC. arsR is a protein that represses the transcription of the ars operon; when bound to arsenic the protein arsR no longer represses the operon and operon is transcribed. The arsD, arsA, arsB, and arsC encode proteins that give E. Coli arsenic resistance. This part consists of only the promoter and the arsR protein-encoding region to be used as an arsenic sensor. | + | This part is a subset of the ars operon, which consists of a promoter and 5 genes: arsR, arsD, arsA, arsB, and arsC. arsR is a protein that represses the transcription of the ars operon; when bound to arsenic the protein arsR no longer represses the operon and operon is transcribed. The arsD, arsA, arsB, and arsC encode proteins that give E. Coli arsenic resistance. This part consists of only the promoter and the arsR protein-encoding region to be used as an arsenic sensor. The part can be placed before any protein coding gene(s). ArsR binds to the promoter and represses transcription of the genes in the operon. When arsenic is present, it binds to the arsR repressor, and transcription of the operon occurs. Therefore, the genes in the operon will only be expressed in the presence of arsenic. For example, to test this part, we placed the arsR promoter and gene before the GFP gene and observed GFP production in the presence of arsenic. |
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Latest revision as of 02:58, 10 December 2016
Arsenic Sensor (arsR operon promoter and arsR gene)
This part is a subset of the ars operon, which consists of a promoter and 5 genes: arsR, arsD, arsA, arsB, and arsC. arsR is a protein that represses the transcription of the ars operon; when bound to arsenic the protein arsR no longer represses the operon and operon is transcribed. The arsD, arsA, arsB, and arsC encode proteins that give E. Coli arsenic resistance. This part consists of only the promoter and the arsR protein-encoding region to be used as an arsenic sensor. The part can be placed before any protein coding gene(s). ArsR binds to the promoter and represses transcription of the genes in the operon. When arsenic is present, it binds to the arsR repressor, and transcription of the operon occurs. Therefore, the genes in the operon will only be expressed in the presence of arsenic. For example, to test this part, we placed the arsR promoter and gene before the GFP gene and observed GFP production in the presence of arsenic.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 159
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]