Difference between revisions of "Part:BBa M50010:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| + | This promoter is inhibited by a protein encoded by the arsR gene, generating a negative feedback loop. When arsenic binds to the arsR protein, the promoter is no longer inhibited, allowing transcription to occur. Transcription of the genes following this promoter indicates the presence of arsenic. | ||
===Source=== | ===Source=== | ||
| − | This part can be found on E. Coli chromosomal and plasmid DNA. | + | This part can be found on E. Coli chromosomal and plasmid DNA. (Escherichia coli K-12 substr. MG1655 arsR. (2016). Ecocyc.org. Retrieved 26 October 2016, from http://ecocyc.org/gene?orgid=ECOLI&id=EG12235#tab=REGULON). |
===References=== | ===References=== | ||
Latest revision as of 03:00, 10 December 2016
Arsenic sensor: ars operon Promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This promoter is inhibited by a protein encoded by the arsR gene, generating a negative feedback loop. When arsenic binds to the arsR protein, the promoter is no longer inhibited, allowing transcription to occur. Transcription of the genes following this promoter indicates the presence of arsenic.
Source
This part can be found on E. Coli chromosomal and plasmid DNA. (Escherichia coli K-12 substr. MG1655 arsR. (2016). Ecocyc.org. Retrieved 26 October 2016, from http://ecocyc.org/gene?orgid=ECOLI&id=EG12235#tab=REGULON).