Difference between revisions of "Part:BBa K2120303"
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'''<Description>''' | '''<Description>''' | ||
− | This part is composed of two elements: | + | <br>This part is composed of two elements: |
− | (1) Arabinose induced promoter pBAD and its inhibitor gene ''araC'' [https://parts.igem.org/Part:BBa_K808000 BBa_K808000].This part contains the promoter as well as the coding sequence for the repressor araC which is transcribed in the opposite direction. (“upstream”) By binding to L(+)-arabinose, araC changes its conformation. This causes the protein to diffuse from the DNA thereby inducing transcription. | + | <br>(1) Arabinose induced promoter pBAD and its inhibitor gene ''araC'' [https://parts.igem.org/Part:BBa_K808000 BBa_K808000].This part contains the promoter as well as the coding sequence for the repressor araC which is transcribed in the opposite direction. (“upstream”) By binding to L(+)-arabinose, araC changes its conformation. This causes the protein to diffuse from the DNA thereby inducing transcription. |
− | (2) Tetracyclines induced promoter pTet's inhibitor gene ''tetR''. | + | <br>(2) Tetracyclines induced promoter pTet's inhibitor gene ''tetR''. |
Latest revision as of 17:04, 22 October 2016
araC-pBAD+B0034+tetR+B0015
<Description>
This part is composed of two elements:
(1) Arabinose induced promoter pBAD and its inhibitor gene araC BBa_K808000.This part contains the promoter as well as the coding sequence for the repressor araC which is transcribed in the opposite direction. (“upstream”) By binding to L(+)-arabinose, araC changes its conformation. This causes the protein to diffuse from the DNA thereby inducing transcription.
(2) Tetracyclines induced promoter pTet's inhibitor gene tetR.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961