Difference between revisions of "Part:BBa K2070004"
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Under the presence of Ecf35 (<partinfo>BBa_K2070009</partinfo>) , the promoter can be activated to generate GFP. | Under the presence of Ecf35 (<partinfo>BBa_K2070009</partinfo>) , the promoter can be activated to generate GFP. | ||
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| − | + | [[File:T--UT-Tokyo--BBa K2070005--4.png|500px]] | |
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| − | + | ==Experimental Validation== | |
| + | [[File:T--UT-Tokyo--BBa K2070005-1.png|thumb|centre|700px| '''Measurement of ECF sigma35 and sigma30 promoter activity.''' Fluorescence/OD600 of a construct containing both an ECF sigma35 generating device and ECF sigma 35 inducible GFP generating device BBa_K2070005 was compared to that containing only either one of the devices. *** for P> 0.001 indicates an extremely significant difference between the induced and uninduced state. ]] | ||
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| + | We have also measured the strength of the activated promoter relative to BBa_J23101. | ||
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| + | [[File:T--UT-Tokyo--BBa K2070005-2.png|centre|thumb|700px| '''Measurement of Activated Sigma30 Promoter Activity in Comparison to Promoter BBa_J23101. '''Both parts are ligated in plasmid pSB3K3. The part containing the constitutive sigma35 generator was double transformed into the cell for measurement. Data represents an average of 3 samples and error bars represent the standard deviation. The promoter in its activated state is measured to have a RPU of 3.2 units. | ||
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Latest revision as of 06:58, 25 October 2016
ecf35 inducible GFP reporter
This part contains an Ecf30 promoter, RBS, GFP and double terminator.
Under the presence of Ecf35 (BBa_K2070009) , the promoter can be activated to generate GFP.
Experimental Validation
Measurement of ECF sigma35 and sigma30 promoter activity. Fluorescence/OD600 of a construct containing both an ECF sigma35 generating device and ECF sigma 35 inducible GFP generating device BBa_K2070005 was compared to that containing only either one of the devices. *** for P> 0.001 indicates an extremely significant difference between the induced and uninduced state.
We have also measured the strength of the activated promoter relative to BBa_J23101.
Measurement of Activated Sigma30 Promoter Activity in Comparison to Promoter BBa_J23101. Both parts are ligated in plasmid pSB3K3. The part containing the constitutive sigma35 generator was double transformed into the cell for measurement. Data represents an average of 3 samples and error bars represent the standard deviation. The promoter in its activated state is measured to have a RPU of 3.2 units.