Difference between revisions of "Part:BBa K945002"
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===Part Contributions=== | ===Part Contributions=== | ||
− | Team Washington 2016 has | + | Team Washington 2016 has removed RFC[10] illegal restriction sites from this part as an improvement to its functionality so that it may be used in other biobricks. The part can be found here: https://parts.igem.org/Part:BBa_K2165004 as part K2165004, along with further documentation and data on it. |
− | as part | + | |
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===Promoter characterization=== | ===Promoter characterization=== |
Latest revision as of 06:14, 19 October 2016
CUP1 yeast promoter
This sequence contains the inducible transcription promoter CUP1, useful for controlled gene expression which is actively enhanced in yeasts in the presence of copper.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 279
Illegal XbaI site found at 517
Illegal SpeI site found at 343
Illegal SpeI site found at 523
Illegal PstI site found at 49 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 343
Illegal SpeI site found at 523
Illegal PstI site found at 49 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 55
Illegal BamHI site found at 510
Illegal XhoI site found at 8 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 279
Illegal XbaI site found at 517
Illegal SpeI site found at 343
Illegal SpeI site found at 523
Illegal PstI site found at 49 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 279
Illegal XbaI site found at 517
Illegal SpeI site found at 343
Illegal SpeI site found at 523
Illegal PstI site found at 49
Illegal NgoMIV site found at 36 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 529
Part Contributions
Team Washington 2016 has removed RFC[10] illegal restriction sites from this part as an improvement to its functionality so that it may be used in other biobricks. The part can be found here: https://parts.igem.org/Part:BBa_K2165004 as part K2165004, along with further documentation and data on it.
Promoter characterization
Team Czech Republic 2015 used this promoter very extensively. As a result, the characterization of this part was improved. See the Experience page for details.
Promoter Strength
Tec-Monterrey Ekam is working on quantitatively characterizing the functionality of this part through the use of BBa_K945004 (GFP) as a reporter, which will be finalized shortly.