Difference between revisions of "Part:BBa J45004"
 
(8 intermediate revisions by 2 users not shown)
Line 2: Line 2:
 
<partinfo>BBa_J45004 short</partinfo>
 
<partinfo>BBa_J45004 short</partinfo>
  
−
Codes for BSMT enzyme to convert benzoic acid/salicylic acid to methyl benzoate and methyl salicylate. This part is phBSMT1 from Petunia x hybrida (GenBank). See part design and discussion for more information.
+
<partinfo>BBa_J45004</partinfo> encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I derived from ''BSMT1'' from ''Petunia x hybrida''. BSMT1 catalyzes the conversion of benzoic acid to methyl benzoate and salicylic acid to methyl salicylate. Methyl benzoate has a floral smell and methyl salicylate has a wintergreen smell.
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
{| border="1" cellpadding="4" cellspacing="0" style="border:#c9c9c9 1px solid; margin: 1em 1em 1em 0; border-collapse: collapse; width:710px" <!-- This line here formats your table for you.  Change the code to change the formatting of your table.-->
 +
| align="center" style="background:#f0f0f0;"|'''Substrate'''
 +
| align="center" style="background:#f0f0f0;"|'''pH optimum'''
 +
| align="center" style="background:#f0f0f0;"|'''Apparent K<sub>m</sub> for Substrate (&mu;M)'''
 +
| align="center" style="background:#f0f0f0;"|'''k<sub>cat</sub> &times; 10<sup>-2</sup> (s<sup>-1</sup>))'''
 +
| align="center" style="background:#f0f0f0;"|'''k<sub>cat</sub>/K<sub>m</sub> (mM<sup>-1</sup>&sdot;s<sup>-1</sup>))'''
 +
|--
 +
|SAM<sub>SA</sub>
 +
|
 +
|1.93 &plusmn; 0.18
 +
|1.45 &plusmn; 0.024
 +
|7.65 &plusmn; 0.618
 +
|--
 +
|SAM<sub>BA</sub>
 +
|
 +
|15.78 &plusmn; 1.34
 +
|0.75 &plusmn; 0.023
 +
|0.48 &plusmn; 0.047
 +
|--
 +
|Salicylic acid
 +
|7.0 to 8.0
 +
|51.55 &plusmn; 2.73
 +
|1.2 &plusmn; 0.16
 +
|0.23 &plusmn; 0.038
 +
|--
 +
|Benzoic acid
 +
|7.5
 +
|1273.2 &plusmn; 37
 +
|0.39 &plusmn; 0.04
 +
|0.003 &plusmn; 0.0003
 +
|}
 +
 +
<biblio>
 +
#Negre-2003 pmid=14630969
 +
</biblio>
  
 
<!-- -->
 
<!-- -->
 +
 +
===Contribution===
 +
 +
Group: Valencia_UPV iGEM 2018
 +
Authors: Adrián Requena Gutiérrez, Carolina Ropero
 +
 +
Summarize: we have created part [https://parts.igem.org/Part:BBa_K2656025 BBa_K2656025]. It is the BSMT1 coding sequence [https://parts.igem.org/Part:BBa_J45004 BBa_J45004] adapted into the Golden Gate assembly method. Thus, it is a basic part compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/Design GoldenBraid 3.0] grammar.
 +
 +
 +
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_J45004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_J45004 SequenceAndFeatures</partinfo>
Line 14: Line 58:
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
 +
 +
 +
 
<partinfo>BBa_J45004 parameters</partinfo>
 
<partinfo>BBa_J45004 parameters</partinfo>
 
<!-- -->
 
<!-- -->

Latest revision as of 16:35, 17 October 2018

SAM:benzoic acid/salicylic acid carboxyl methyltransferase I; converts salicylic acid to methyl sali

BBa_J45004 encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I derived from BSMT1 from Petunia x hybrida. BSMT1 catalyzes the conversion of benzoic acid to methyl benzoate and salicylic acid to methyl salicylate. Methyl benzoate has a floral smell and methyl salicylate has a wintergreen smell.

Usage and Biology

Substrate pH optimum Apparent Km for Substrate (μM) kcat × 10-2 (s-1)) kcat/Km (mM-1⋅s-1))
SAMSA 1.93 ± 0.18 1.45 ± 0.024 7.65 ± 0.618
SAMBA 15.78 ± 1.34 0.75 ± 0.023 0.48 ± 0.047
Salicylic acid 7.0 to 8.0 51.55 ± 2.73 1.2 ± 0.16 0.23 ± 0.038
Benzoic acid 7.5 1273.2 ± 37 0.39 ± 0.04 0.003 ± 0.0003

<biblio>

  1. Negre-2003 pmid=14630969

</biblio>


Contribution

Group: Valencia_UPV iGEM 2018 Authors: Adrián Requena Gutiérrez, Carolina Ropero

Summarize: we have created part BBa_K2656025. It is the BSMT1 coding sequence BBa_J45004 adapted into the Golden Gate assembly method. Thus, it is a basic part compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/Design GoldenBraid 3.0] grammar.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 663
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 204