Difference between revisions of "Part:BBa K2070004"
 
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This part contains the ecf16 promoter,RBS,GFP and double terminator.
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This part contains an Ecf30 promoter, RBS, GFP and double terminator.
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Under the presence of ecf16, the promoter can be activated to generate GFP.  
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Under the presence of Ecf35 (<partinfo>BBa_K2070009</partinfo>) , the promoter can be activated to generate GFP.  
  
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
  
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[[File:T--UT-Tokyo--BBa K2070005--4.png|500px]]
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2070004 SequenceAndFeatures</partinfo>
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==Experimental Validation==
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[[File:T--UT-Tokyo--BBa K2070005-1.png|thumb|centre|700px| '''Measurement of ECF sigma35 and sigma30 promoter activity.''' Fluorescence/OD600 of a construct containing both an ECF sigma35 generating device and ECF sigma 35 inducible GFP generating device BBa_K2070005 was compared to that containing only either one of the devices. *** for P> 0.001 indicates an extremely significant difference between the induced and uninduced state. ]]
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We have also measured the strength of the activated promoter relative to BBa_J23101.
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[[File:T--UT-Tokyo--BBa K2070005-2.png|centre|thumb|700px| '''Measurement of Activated Sigma30 Promoter Activity in Comparison to Promoter BBa_J23101. '''Both parts are ligated in plasmid pSB3K3. The part containing the constitutive sigma35 generator was double transformed into the cell for measurement. Data represents an average of 3 samples and error bars represent the standard  deviation. The promoter in its activated state is measured to have a RPU of 3.2 units.
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Latest revision as of 06:58, 25 October 2016


ecf35 inducible GFP reporter


This part contains an Ecf30 promoter, RBS, GFP and double terminator. Under the presence of Ecf35 (BBa_K2070009) , the promoter can be activated to generate GFP.


T--UT-Tokyo--BBa K2070005--4.png

Experimental Validation

Measurement of ECF sigma35 and sigma30 promoter activity. Fluorescence/OD600 of a construct containing both an ECF sigma35 generating device and ECF sigma 35 inducible GFP generating device BBa_K2070005 was compared to that containing only either one of the devices. *** for P> 0.001 indicates an extremely significant difference between the induced and uninduced state.

We have also measured the strength of the activated promoter relative to BBa_J23101.

Measurement of Activated Sigma30 Promoter Activity in Comparison to Promoter BBa_J23101. Both parts are ligated in plasmid pSB3K3. The part containing the constitutive sigma35 generator was double transformed into the cell for measurement. Data represents an average of 3 samples and error bars represent the standard deviation. The promoter in its activated state is measured to have a RPU of 3.2 units.