Difference between revisions of "Part:BBa K2120303"

m
 
(2 intermediate revisions by the same user not shown)
Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2120303 short</partinfo>
 
<partinfo>BBa_K2120303 short</partinfo>
−
pBAD is a promoter induced by arabinose and arac is the repressor. We use it to test the expression of reporter gene in our validation circuit .And tetR is an inhibitor which is built to repress the expression of Ptet promoter. We use it to control the “on” or ”off” state of toxin gene.
+
 
 +
'''<Description>'''
 +
<br>This part is composed of two elements:
 +
<br>(1) Arabinose induced promoter pBAD and its inhibitor gene ''araC'' [https://parts.igem.org/Part:BBa_K808000 BBa_K808000].This part contains the promoter as well as the coding sequence for the repressor araC which is transcribed in the opposite direction. (“upstream”) By binding to L(+)-arabinose, araC changes its conformation. This causes the protein to diffuse from the DNA thereby inducing transcription.
 +
<br>(2) Tetracyclines induced promoter pTet's inhibitor gene ''tetR''.
 +
 
  
  

Latest revision as of 17:04, 22 October 2016


araC-pBAD+B0034+tetR+B0015

<Description>
This part is composed of two elements:
(1) Arabinose induced promoter pBAD and its inhibitor gene araC BBa_K808000.This part contains the promoter as well as the coding sequence for the repressor araC which is transcribed in the opposite direction. (“upstream”) By binding to L(+)-arabinose, araC changes its conformation. This causes the protein to diffuse from the DNA thereby inducing transcription.
(2) Tetracyclines induced promoter pTet's inhibitor gene tetR.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961