Difference between revisions of "Part:BBa K1616005"
 
(3 intermediate revisions by 2 users not shown)
Line 7: Line 7:
 
<br>
 
<br>
 
<h3> <font style="color:#b22222">Reverse sequence</font> </h3>
 
<h3> <font style="color:#b22222">Reverse sequence</font> </h3>
 +
Our kill switch was designed to work in a system like pDawn, a light-inducible expression system. However, this system works with reversed sequence. That is why we designed a simple reversed Biobrick Holin-Endolysin (HE). This part was inserted into native pDawn for characterization of the Biobrick.
 
This composite part is composed of the reverse sequence of Holin and the reverse sequence of Endolysin.  
 
This composite part is composed of the reverse sequence of Holin and the reverse sequence of Endolysin.  
  
 +
<br>
 +
[[Image:Part toxin holin endolysine reverse.png|center|600px|thumb|'''Fig. 1:'''  Holin - Endolysin construction]] 
  
  
−
 
+
<br>
−
 
+
−
 
+
−
 
+
 
<partinfo>BBa_K1616005 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1616005 SequenceAndFeatures</partinfo>
  

Latest revision as of 23:12, 22 September 2015

Holin/endolysin reversed - RBS and double T7 terminator

Holin - Endolysin

Holin and endolysin are toxins; after a period of late-gene expression, holin, an inner permeabilizing protein creates holes in the membrane in order to permeabilize it and then endolysin, a muralytic enzyme traverses the holes creating by holin and degraded the wall cell. This complex induce cell lysis of bacteria. (Young & Bläsi, 1995)

Reverse sequence

Our kill switch was designed to work in a system like pDawn, a light-inducible expression system. However, this system works with reversed sequence. That is why we designed a simple reversed Biobrick Holin-Endolysin (HE). This part was inserted into native pDawn for characterization of the Biobrick. This composite part is composed of the reverse sequence of Holin and the reverse sequence of Endolysin.


Fig. 1: Holin - Endolysin construction




Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 306
    Illegal AgeI site found at 376
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

All BioBrick parts used for assembling the composite part are compatible with the RFC10 Biobrick standard.


Source

BBa_K1616005 is a composite part with BBa_K1616006 (endolysin reversed), BBa_K1616007 (reversed), BBa_K1616011 (RBS reversed) and BBa_B0025 (double terminator T7 reversed).

References

Young, R., & Bläsi, U. (1995). Holins: Form and function in bacteriophage lysis. In FEMS Microbiology Reviews (Vol. 17, pp. 191–205). http://doi.org/10.1016/01686445(94)00079-4