Difference between revisions of "Part:BBa K1777005"

Latest revision as of 16:08, 20 September 2015

flexible linker with SV40 NLS

((natural flexible linker)-(SV40 NLS)-(artificial GS linker))2
Function	Protein linker
Use in	Mammalian cells
RFC standard	RFC 10
Backbone	pSB1C3
Submitted by	[http://2015.igem.org/Team:Fudan Fudan 2015]

This is an improved flexible linker offering around 50 Angstrom separation for fusion domains.Specially, this linker was designed with a SV40 NLS, and its codon is optimized for mammalian.So it can be well used to link different protein domains for fusion proteins locating in cell nucleus.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1777005 short</partinfo>
+{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
+! colspan="2" style="background:#FFBF00;"|((natural flexible linker)-(SV40 NLS)-(artificial GS linker))2
+|-
+|'''Function'''
+|Protein linker
+|-
+|'''Use in'''
+|Mammalian cells
+|-
+|'''RFC standard'''
+|[https://parts.igem.org/Help:Assembly_standard_10 RFC 10]
+|-
+|'''Backbone'''
+|pSB1C3<br>
+|-
+|'''Submitted by'''
+|[http://2015.igem.org/Team:Fudan Fudan 2015]
+|}
 This is an improved flexible linker offering around 50 Angstrom separation for fusion domains.Specially, this linker was designed with a SV40 NLS, and its codon is optimized for mammalian.So it can be well used to link different protein domains for fusion proteins locating in cell nucleus.