Difference between revisions of "Part:BBa K1638010:Design"
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
Latest revision as of 12:56, 18 September 2015
T25 domain of cyaA from Bordetella pertussis with GFP reporter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 843
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1516
Design Notes
This part was assemblied by the part containing the T25 domain (BBa_K1638003) and the linker-GFP (BBa_K1638007). These parts respectively contains a BamHI res. site in the 3'-end and the 5'-end allowing assembly without creating a scarsite. Scarsite includes a stop-codon, which is not favorable for correct of assembly of fusion proteins.
Source
pKT25
BBa_E0040