Difference between revisions of "Part:BBa K1638007"
 
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<partinfo>BBa_K1638007 short</partinfo>
 
<partinfo>BBa_K1638007 short</partinfo>
  
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This part codes for a green fluorescent protein (GFP) reporter with a flexible 10 aa linker at N-terminus. Intended for use in protein-GFP fusions, where this part can be suffixed to various proteins. The protein-GFP fusion can be used as a reporter of gene expression and used in localization assays. A TEV-protease recognition site enables separation of the two proteins. This can be useful in different occasions, e.g. protein purification.
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This part codes for a green fluorescent protein (GFP) reporter with a flexible 10 aa linker at N-terminus. Intended for use in protein-GFP fusions, where this part can be suffixed to various proteins. The protein-GFP fusion can be used as a reporter of gene expression and used in localization assays. A Tobacco etch virus (TEV) protease recognition site, ENLYFQS, enables separation of the two proteins. This can be useful in different occasions, e.g. protein purification.
 
This part also contains two transcriptional terminators.
 
This part also contains two transcriptional terminators.
  
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*'''Emission max''' - 511nm
 
*'''Emission max''' - 511nm
  
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For further description of the GFP [https://parts.igem.org/wiki/index.php/Part:BBa_E0040 read here].
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For a more detailed description of this green fluorescent protein, read here: <partinfo>BBa_E0040</partinfo>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 13:01, 18 September 2015

GFP reporter with flexible linker

This part codes for a green fluorescent protein (GFP) reporter with a flexible 10 aa linker at N-terminus. Intended for use in protein-GFP fusions, where this part can be suffixed to various proteins. The protein-GFP fusion can be used as a reporter of gene expression and used in localization assays. A Tobacco etch virus (TEV) protease recognition site, ENLYFQS, enables separation of the two proteins. This can be useful in different occasions, e.g. protein purification. This part also contains two transcriptional terminators.

Fluorescence wavelengths

  • Excitation max - 501nm
  • Emission max - 511nm

For a more detailed description of this green fluorescent protein, read here: BBa_E0040

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 674