Difference between revisions of "Part:BBa K1602040"

 
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1602040 short</partinfo>
 
<partinfo>BBa_K1602040 short</partinfo>
  
 +
<html>
 +
    <head>
 +
        <style>
 +
            table, td, th { border: 0px solid black; }
 +
        </style>
 +
    </head>
 +
    <body>
 +
   
 +
        <table>
 +
            <colgroup width=50% span="2">
 +
            <tr>
 +
                <td>
 +
                This part is capable to generate the enhanced yellow fluorescent protein <b>(eYFP) </b> codon optimized for usage in <i>E. coli</i> (<a href="https://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>) and under control of a T7-promotor. To fuse the protein to an <i>in vitro</i> scaffold (<a href="https://parts.igem.org/Part:BBa_K1602027">BBa_K1602027</a>) a linker-ligand construct was added. This ligand binds to the GBD domain of the scaffold.
 +
                </td>
 +
               
 +
            </tr>
 +
            <tr>
 +
                <td>
 +
                    <!-- BLANK -->
 +
                </td>
 +
         
 +
            </tr>
 +
            </colgroup>
 +
        </table>
 +
    </body>
 +
</html>
  
  

Latest revision as of 02:56, 19 September 2015

Inducible generator of eYFP with C-terminal GBD ligand

This part is capable to generate the enhanced yellow fluorescent protein (eYFP) codon optimized for usage in E. coli (BBa_B0034) and under control of a T7-promotor. To fuse the protein to an in vitro scaffold (BBa_K1602027) a linker-ligand construct was added. This ligand binds to the GBD domain of the scaffold.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]