Difference between revisions of "Part:BBa K1602014"

 
 
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        <h1>B0034-yagE</h1>
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        This composite represents the  2-dehydro-3-deoxy-<small>D</small>-pentonate aldolases <i>yagE</i> plus the ribosomal binding site B0034.
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===<h2>Usage</h2>===
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        This part is a composite of one coding gene and a strong RBS (<a href="/Part:BBa_B0034">BBa_B0034</a>).
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        This part makes it possible to establish an ethyleneglycol producing pathway in other hosts than <i>E. coli</i> if its combined with other enzymes of the pathway <a href="https://parts.igem.org/Part:BBa_K1602017">(BBa_K1602017)</a> <a href="https://parts.igem.org/Part:BBa_K1602002">(BBa_K1602002)</a> <a href="https://parts.igem.org/Part:BBa_K1602015">(BBa_K1602015)</a>. (1)
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                        <li>yagE</li>
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                        <li>RBS-B0034</li>
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                        <li><a href="https://parts.igem.org/Part:BBa_K1602011">BBa_K1602011</a></li>
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                        <li><a href="https://parts.igem.org/Part:BBa_B0034">BBa_B0034</a></li>
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                    <div align="center" valign="middle"><img class="transparent shrinkToFit" alt="https://static.igem.org/mediawiki/parts/8/8e/TU_Darmstadt_plasmid-card_B0034-yagE.png" src="https://static.igem.org/mediawiki/parts/8/8e/TU_Darmstadt_plasmid-card_B0034-yagE.png" height="227" width="255"></div>
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===<h2>Sequence and Features</h2>===
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<partinfo>BBa_K1602014 short</partinfo>
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<partinfo>BBa_K1602014 SequenceAndFeatures</partinfo>
  
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===References===
  
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1. Liu H, Ramos KR, Valdehuesa KN, Nisola GM, Lee WK, Chung WJ. Biosynthesis of ethylene glycol in Escherichia coli. Appl Microbiol Biotechnol. 2013;97(8):3409-17.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1602014 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K1602014 parameters</partinfo>
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Latest revision as of 22:37, 18 September 2015

B0034-yagE

This composite represents the 2-dehydro-3-deoxy-D-pentonate aldolases yagE plus the ribosomal binding site B0034.

Usage

This part is a composite of one coding gene and a strong RBS (BBa_B0034).

This part makes it possible to establish an ethyleneglycol producing pathway in other hosts than E. coli if its combined with other enzymes of the pathway (BBa_K1602017) (BBa_K1602002) (BBa_K1602015). (1)

  • yagE
  • RBS-B0034
https://static.igem.org/mediawiki/parts/8/8e/TU_Darmstadt_plasmid-card_B0034-yagE.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 193


References

1. Liu H, Ramos KR, Valdehuesa KN, Nisola GM, Lee WK, Chung WJ. Biosynthesis of ethylene glycol in Escherichia coli. Appl Microbiol Biotechnol. 2013;97(8):3409-17.