Difference between revisions of "Part:BBa K1777003"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1777003 short</partinfo>
 
<partinfo>BBa_K1777003 short</partinfo>
  
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This part has 6 mir-21 binding site and is flanked by bate-globing intron1 splicing site. This parts can sequester mir-21. Add cyclizing device to the flanking region of this part, it can be involved into a back-splicing mechanism and become a circRNA sponge.
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{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
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! colspan="2" style="background:#FFBF00;"|(β-globin SA)-(miR-21 binding site)6-(β-globin SD)
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|-
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|'''Function'''
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|Linear miR-21 sponge
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|-
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|'''Use in'''
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|Mammalian cells
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|-
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|'''RFC standard'''
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|[https://parts.igem.org/Help:Assembly_standard_10 RFC 10]
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|-
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|'''Backbone'''
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|pSB1C3<br>
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|-
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|'''Submitted by'''
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|[http://2015.igem.org/Team:Fudan Fudan 2015]
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|}
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The Fudan iGEM team 2015 designed the part which has 6 miR-21 binding sites and is flanked by β-globing intron1 splicing site. We used it as the linear miR-21 sponge to sequester miR-21, compared with the blank control groups and circRNA sponge groups. In addition, adding cyclizing device to the flanking region of this part, it can be involved into a back-splicing mechanism and become a circRNA sponge.<br>
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As the controls of blank groups, the linear miR-21 sponges have the similiar constructs with "Ouroboros"([https://parts.igem.org/Part:BBa_K1777016 Part:BBa_K1777016]) and only lack inverted repeat sequences.<br>
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[[File: Overview_of_the_construct_linear_miR-21_sponge_igem_2015.png| 700 px | center]]
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'''Figure 1: Construct design.''' <br>
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[[File: ls.jpg| 700px | center]]<br>
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'''Figure 2: Examination of efficiency of miR-21 sponges.'''<br>
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At an appropriate molar ratio of luciferase tools and sponges,the expression of ''Renilla'' shall increase.<br>
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===Proof of function===
  
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1777003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1777003 SequenceAndFeatures</partinfo>

Latest revision as of 22:52, 23 September 2015

miR-21 linear sponge

(β-globin SA)-(miR-21 binding site)6-(β-globin SD)
Function Linear miR-21 sponge
Use in Mammalian cells
RFC standard RFC 10
Backbone pSB1C3
Submitted by [http://2015.igem.org/Team:Fudan Fudan 2015]

The Fudan iGEM team 2015 designed the part which has 6 miR-21 binding sites and is flanked by β-globing intron1 splicing site. We used it as the linear miR-21 sponge to sequester miR-21, compared with the blank control groups and circRNA sponge groups. In addition, adding cyclizing device to the flanking region of this part, it can be involved into a back-splicing mechanism and become a circRNA sponge.
As the controls of blank groups, the linear miR-21 sponges have the similiar constructs with "Ouroboros"(Part:BBa_K1777016) and only lack inverted repeat sequences.

Overview of the construct linear miR-21 sponge igem 2015.png

Figure 1: Construct design.

Ls.jpg

Figure 2: Examination of efficiency of miR-21 sponges.
At an appropriate molar ratio of luciferase tools and sponges,the expression of Renilla shall increase.

Proof of function

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 81
    Illegal XhoI site found at 452
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 423