Difference between revisions of "Part:BBa K1583014"

 
 
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<partinfo>BBa_K1583014 short</partinfo>
 
<partinfo>BBa_K1583014 short</partinfo>
  
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This part was designed to create a fusion protein of CsgA with Mfp3 (mussel foot protein). This protein has got high adhesive properties towards wet polar surfaces. Like this, an amyloid nanowire with high binding properties is created.
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<p>In order to create a fusion protein of CsgA (<a href="https://parts.igem.org/Part:BBa_K1583000"target="_blank">BBa_K158300</a>) with Mfp3 (<a href="https://parts.igem.org/Part:BBa_K1583003"target="_blank">BBa_K1583003</a>) at the C-terminus. CsgA was therefore designed without its stop codon.</p>
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><h3>Sequence and Features</h3></span>
 
<partinfo>BBa_K1583014 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1583014 SequenceAndFeatures</partinfo>
  

Latest revision as of 00:59, 19 September 2015

CsgA missing the stop codon for creation of fusion proteins

In order to create a fusion protein of CsgA (BBa_K158300) with Mfp3 (BBa_K1583003) at the C-terminus. CsgA was therefore designed without its stop codon.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]