Difference between revisions of "Part:BBa K1758101:Design"

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*Part one:
 
*Part one:
 
'''Forward (split) primer [http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#Cm_fwd Cm_fwd]'''
 
'''Forward (split) primer [http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#Cm_fwd Cm_fwd]'''
 +
 
'''[http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#UTR_noT7_rev UTR_noT7_rev]'''
 
'''[http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#UTR_noT7_rev UTR_noT7_rev]'''
  
 
*Part two:
 
*Part two:
 
'''Reverse(split) primer [http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#Cm_rev Cm_rev]'''
 
'''Reverse(split) primer [http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#Cm_rev Cm_rev]'''
 +
 
'''[http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#UTR_fwd UTR_fwd]
 
'''[http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#UTR_fwd UTR_fwd]
  
 
===References===
 
===References===

Latest revision as of 23:33, 21 August 2015

Translation enhancing 5-UTR + sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 59


Design Notes

[http://www.ncbi.nlm.nih.gov/pubmed/23654270 Lentini et al. 2013] showed that the scar which is created by standard biobrick assembly is disadvantageous for in vitro translation when occuring between RBS and start codon. Therefore we changed the sequence between RBS and start codon for other parts.

Source

This part was created by shortening BBa_I746909 and simultaneously adding 5'-UTR via Gibson primers.

Amplification with Gibson-primers:

  • Part one:

Forward (split) primer [http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#Cm_fwd Cm_fwd]

[http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#UTR_noT7_rev UTR_noT7_rev]

  • Part two:

Reverse(split) primer [http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#Cm_rev Cm_rev]

[http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#UTR_fwd UTR_fwd]

References