Difference between revisions of "Part:BBa J45120"
 
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<partinfo>BBa_J45120 short</partinfo>
 
<partinfo>BBa_J45120 short</partinfo>
  
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This is the wintergreen generating device hooked up to a constitutive promoter with a different RBS (B0032, different from 45100).  This part is almost identical to <partinfo>J45100</partinfo>, except that it uses the B0032 RBS instead of B0030 (which is in <partinfo>J45100</partinfo>).
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The wintergreen odor generator (<partinfo>BBa_J45120</partinfo>) catalyzes the conversion of the precursor salicylic acid to the odor methyl salicylate that has a wintergreen smell.  The biosynthetic device is composed of two transcriptional devices: a constitutive transcription source (<partinfo>BBa_R0040</partinfo>) and an odor enzyme generator (<partinfo>BBa_J45119</partinfo>). Odor enzyme generators take as input a transcriptional signal and produce as output an enzyme that catalyzes production of an odor from a chemical precursor. 
  
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[[Image:BBa J45120.png|center]]
  
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===Usage and Biology===
 
===Usage and Biology===
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*Supplement cultures with 2 mM salicylic acid to produce wintergreen odor.
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*See <partinfo>BBa_J45004</partinfo> for details.
  
 
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Latest revision as of 23:49, 8 March 2008

Wintergreen odor generator

The wintergreen odor generator (BBa_J45120) catalyzes the conversion of the precursor salicylic acid to the odor methyl salicylate that has a wintergreen smell. The biosynthetic device is composed of two transcriptional devices: a constitutive transcription source (BBa_R0040) and an odor enzyme generator (BBa_J45119). Odor enzyme generators take as input a transcriptional signal and produce as output an enzyme that catalyzes production of an odor from a chemical precursor.

BBa J45120.png

Usage and Biology

  • Supplement cultures with 2 mM salicylic acid to produce wintergreen odor.
  • See BBa_J45004 for details.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 744
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 285