Difference between revisions of "Part:BBa Q04401:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_Q04401=== | ===Applications of BBa_Q04401=== | ||
− | * Used in | + | *Used successfully as a transcriptional inverter in the exponential phase GFP generator (<partinfo>BBa_J45996</partinfo>). See results below. |
===User Reviews=== | ===User Reviews=== | ||
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<!-- End of the user review template --> | <!-- End of the user review template --> | ||
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+ | {|width='80%' style='border:1px solid gray' | ||
+ | |- | ||
+ | |width='10%'| | ||
+ | <partinfo>BBa_Q04401 AddReview 3</partinfo> | ||
+ | <I>[[User:Jasonk|Jason Kelly]]</I> | ||
+ | |width='60%' valign='top'| | ||
+ | In general, I think it's more likely to work when used on a high-copy (1 series) plasmid. I've tried it on a 3 series, using <bbpart>BBa_F2621</bbpart>as the induction system and did not see flipping of the inverter. | ||
+ | |} | ||
+ | |||
+ | {|width='80%' style='border:1px solid gray' | ||
+ | |- | ||
+ | |width='10%'| | ||
+ | <partinfo>BBa_Q04401 AddReview 4</partinfo> | ||
+ | <I>[[User:Rshetty|Reshma Shetty]]</I> | ||
+ | |width='60%' valign='top'| | ||
+ | <partinfo>BBa_Q04401</partinfo> worked well in the exponential phase GFP generator (<partinfo>BBa_J45996</partinfo>). | ||
+ | |} | ||
+ | |||
<!-- DON'T DELETE --><partinfo>BBa_Q04401 EndReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_Q04401 EndReviews</partinfo> | ||
+ | |||
+ | ==Characterization== | ||
+ | |||
+ | ===Transfer curve=== | ||
+ | [[Image:Q04401.JPG|thumb|500px|left| | ||
+ | |||
+ | '''Transfer curve for <partinfo>BBa_Q04401</partinfo> measured in the Screening Plasmid (<partinfo>pSB1A10</partinfo>).'''<br> | ||
+ | |||
+ | Units are arbitrary. The image in top right is a dot plot based on concatenated flow cytometry data from 6 different arabinose induction levels (see <partinfo>pSB1A10</partinfo>for details). Main figure is the same dot plot data averaged to make the transfer curve more clear.]] | ||
+ | |||
+ | <br style="clear:both" /> | ||
+ | |||
+ | ===Use in <partinfo>BBa_J45996</partinfo>: an exponential phase GFP generator composed of <partinfo>BBa_J45992</partinfo>, <partinfo>BBa_Q04401</partinfo> and <partinfo>BBa_E0840</partinfo>=== | ||
+ | |||
+ | {{:iGEM:MIT/2006/Transcriptional control devices}} | ||
+ | |||
+ | <br style="clear:both;"/> |
Latest revision as of 01:26, 8 March 2008
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_Q04401
- Used successfully as a transcriptional inverter in the exponential phase GFP generator (BBa_J45996). See results below.
User Reviews
UNIQ20c7dda82c2a5daf-partinfo-00000001-QINU
•••
|
In general, I think it's more likely to work when used on a high-copy (1 series) plasmid. I've tried it on a 3 series, using BBa_F2621as the induction system and did not see flipping of the inverter. |
••••
|
BBa_Q04401 worked well in the exponential phase GFP generator (BBa_J45996). |
UNIQ20c7dda82c2a5daf-partinfo-00000007-QINU
Characterization
Transfer curve
Use in BBa_J45996: an exponential phase GFP generator composed of BBa_J45992, BBa_Q04401 and BBa_E0840