Difference between revisions of "Part:BBa J45200"
 
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<partinfo>BBa_J45200 short</partinfo>
 
<partinfo>BBa_J45200 short</partinfo>
  
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Protein generator producing the protein ATF1 under control of the LacI repressor.  Thus, in a LacI<sup>-</sup> ''E. coli'' strain, ATF1 is continually produced.  ATFI encodes the enzyme alcohol acetyltransferase I (AATase I), which produces acetate esters from acetyl CoA and alcohols (including isoamyl acetate from isoamyl alcohol).  This part is almost identical to <partinfo>J45220</partinfo> except that it uses the B0030 RBS (instead of B0032 as is in <partinfo>J45220</partinfo>).  The Banana Generating Device (BGD) function thus far has been to convert isoamyl alcohol into isoamyl acetate (a banana-scented compound).
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The banana odor generator (<partinfo>BBa_J45200</partinfo>) catalyzes the conversion of the precursor isoamyl alcohol to the odor isoamyl acetate that has a banana smell.  The biosynthetic device is composed of two transcriptional devices: a constitutive transcription source (<partinfo>BBa_R0040</partinfo>) and an odor enzyme generator (<partinfo>BBa_J45199</partinfo>).  Odor enzyme generators take as input a transcriptional signal and produce as output an enzyme that catalyzes production of an odor from a chemical precursor. 
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[[Image:BBa_J45200.png|center]]
  
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===Usage and Biology===
 
===Usage and Biology===
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*Supplement cultures with 5 mM isoamyl alcohol to produce banana odor.
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*See <partinfo>BBa_J45014</partinfo> for details.
  
 
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Latest revision as of 23:49, 8 March 2008

Banana odor generator

The banana odor generator (BBa_J45200) catalyzes the conversion of the precursor isoamyl alcohol to the odor isoamyl acetate that has a banana smell. The biosynthetic device is composed of two transcriptional devices: a constitutive transcription source (BBa_R0040) and an odor enzyme generator (BBa_J45199). Odor enzyme generators take as input a transcriptional signal and produce as output an enzyme that catalyzes production of an odor from a chemical precursor.

BBa J45200.png

Usage and Biology

  • Supplement cultures with 5 mM isoamyl alcohol to produce banana odor.
  • See BBa_J45014 for details.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 182
    Illegal BamHI site found at 1509
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 609
    Illegal SapI.rc site found at 1617