Difference between revisions of "Part:BBa J45009"
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<partinfo>BBa_J45009 short</partinfo> | <partinfo>BBa_J45009 short</partinfo> | ||
− | + | <partinfo>BBa_J45009</partinfo> encodes a branched-chain amino acid transaminase derived from the ''THI3'' gene from ''Saccharomyces cerevisiae''. THI3 catalyzes the second step in isoamyl alcohol biosynthesis: conversion of α-ketoisocaproate to 3-methylbutanal via enzymatic reaction 4.1.1.1. | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | *''THI3'' is formerly known as ''KID1''. | ||
+ | *3-methylbutanal is reduced via a chassis alcohol dehydrogenase to isoamyl alcohol which is a precursor to isoamyl acetate (banana odor). | ||
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+ | *'''Note: <partinfo>BBa_J45009</partinfo> has several internal BioBrick restriction sites. We indicate proposed silent mutations for eliminating the BioBrick restriction sites. It can only be used as a suffix part in BioBrick assembly.''' | ||
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Latest revision as of 22:43, 7 March 2008
ketoisocaproate decarboxylase (THI3); converts alpha-ketoisocaproate to 3-methylbutanal
BBa_J45009 encodes a branched-chain amino acid transaminase derived from the THI3 gene from Saccharomyces cerevisiae. THI3 catalyzes the second step in isoamyl alcohol biosynthesis: conversion of α-ketoisocaproate to 3-methylbutanal via enzymatic reaction 4.1.1.1.
Usage and Biology
- THI3 is formerly known as KID1.
- 3-methylbutanal is reduced via a chassis alcohol dehydrogenase to isoamyl alcohol which is a precursor to isoamyl acetate (banana odor).
- Note: BBa_J45009 has several internal BioBrick restriction sites. We indicate proposed silent mutations for eliminating the BioBrick restriction sites. It can only be used as a suffix part in BioBrick assembly.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 3
Illegal EcoRI site found at 904
Illegal EcoRI site found at 1603
Illegal EcoRI site found at 1789
Illegal SpeI site found at 697 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3
Illegal EcoRI site found at 904
Illegal EcoRI site found at 1603
Illegal EcoRI site found at 1789
Illegal SpeI site found at 697 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3
Illegal EcoRI site found at 904
Illegal EcoRI site found at 1603
Illegal EcoRI site found at 1789 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 3
Illegal EcoRI site found at 904
Illegal EcoRI site found at 1603
Illegal EcoRI site found at 1789
Illegal SpeI site found at 697 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 3
Illegal EcoRI site found at 904
Illegal EcoRI site found at 1603
Illegal EcoRI site found at 1789
Illegal SpeI site found at 697 - 1000COMPATIBLE WITH RFC[1000]