Difference between revisions of "Part:BBa K1319014"
 
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<partinfo>BBa_K1319014 short</partinfo>
 
<partinfo>BBa_K1319014 short</partinfo>
  
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This part expresses a [https://parts.igem.org/Part:BBa_E0040 E0040].[https://parts.igem.org/Part:BBa_K1319002 K1319002] fusion protein (wtGFP-REACh1) behind a [https://parts.igem.org/Part:BBa_J23101 J23101] promoter.  
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This part expresses a [https://parts.igem.org/Part:BBa_E0040 E0040].[https://parts.igem.org/Part:BBa_K1319002 K1319002] fusion protein (GFP-REACh1) behind a [https://parts.igem.org/Part:BBa_J23101 J23101] promoter.  
 
[https://parts.igem.org/Part:BBa_K1319002 K1319002] is a '''dark quencher''' that eliminates the fluorescence of the GFP-domain by Förster Resonance Energy Transfer (FRET), but does not exhibit strong fluorescence itself.
 
[https://parts.igem.org/Part:BBa_K1319002 K1319002] is a '''dark quencher''' that eliminates the fluorescence of the GFP-domain by Förster Resonance Energy Transfer (FRET), but does not exhibit strong fluorescence itself.
  
 
===Usage and Biology===
 
===Usage and Biology===
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The two domains can be separated from each other via a TEV protease cleavage site in the linker.
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More information about the characterization of this part can be found on the part page of [https://parts.igem.org/Part:BBa_K1319002 K1319002].
 
More information about the characterization of this part can be found on the part page of [https://parts.igem.org/Part:BBa_K1319002 K1319002].
  

Latest revision as of 02:22, 18 October 2014

Constitutive expression of GFP-REACh2 fusion protein

This part expresses a E0040.K1319002 fusion protein (GFP-REACh1) behind a J23101 promoter. K1319002 is a dark quencher that eliminates the fluorescence of the GFP-domain by Förster Resonance Energy Transfer (FRET), but does not exhibit strong fluorescence itself.

Usage and Biology

The two domains can be separated from each other via a TEV protease cleavage site in the linker.

More information about the characterization of this part can be found on the part page of K1319002.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1521
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 706