Difference between revisions of "Part:BBa K1381006"

Latest revision as of 15:22, 16 October 2014

J23102-B0034-YenR

This construct was created to characterise BBa_K1381004, the activator YenR. YenR is an activator originated from Yersinia enterocolitica. When YenR binds to the recognition region, BBa_K1381000 (the yenbox), the expression level of the fused promoter is induced. Both YenR and the yenbox are parts in the Yen system, which is a homolougous system to the Lux system. [1]

The BioBricks that were used for assembling this construct was the promoter BBa_J23102 (J23102) from the Anderson promoter library and the activator in the Yen system BBa_K1381004 (B0034-YenR). To read more about the Yen system and for the results from the characterisation experiments, see BBa_K1381004 (B0034-YenR).

References
[1] Ching-Sung Tsai and Stephen C. Winanas, The quorum-hindered transcription factor YenR of Yersinia enterocolitica inhibits pheromone production and promotes motility via a small non-coding RNA, 2011, Molecular Microbiology 80[2], 556-571

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 This construct was created to characterise [https://parts.igem.org/Part:BBa_K1381004 BBa_K1381004], the activator YenR. YenR is an activator originated from <i>Yersinia enterocolitica</i>. When YenR binds to the recognition region, [https://parts.igem.org/Part:BBa_K1381000 BBa_K1381000] (the yenbox), the expression level of the fused promoter is induced. Both YenR and the yenbox are parts in the Yen system, which is a homolougous system to the Lux system. [1]
-The BioBrick that were used for assembling this construct was the promoter [https://parts.igem.org/Part:BBa_J23110 BBa_J23102] (J23102) from the Anderson promoter library and the activator in the Yen system [https://parts.igem.org/Part:BBa_K1381004 BBa_K1381004] (B0034-YenR).
+The BioBricks that were used for assembling this construct was the promoter [https://parts.igem.org/Part:BBa_J23102 BBa_J23102] (J23102) from the Anderson promoter library and the activator in the Yen system [https://parts.igem.org/Part:BBa_K1381004 BBa_K1381004] (B0034-YenR).
-Too read more about the Yen system and for the results from the characterisation experiments, see [https://parts.igem.org/Part:BBa_K1381004 BBa_K1381004] (B0034-YenR).
+To read more about the Yen system and for the results from the characterisation experiments, see [https://parts.igem.org/Part:BBa_K1381004 BBa_K1381004] (B0034-YenR).