Difference between revisions of "Part:BBa K1465101:Design"

 
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===Design Notes===
 
===Design Notes===
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BB
 
  
  
 +
*Amplification of the pSB1C3-Backbone (<bbpart>BBa_J04450</bbpart>) using the specific Gibson-Primer [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_pre_pccH pSB1C3_pre_pccH] and [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_suf_pccH pSB1C3_suf_pccH]:<br>
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'''pSB1C3_pre_pccH (58 bp)'''<br>
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5'-GAAAGAATAGTTGTTTTTTGCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3'<br>
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'''pSB1C3_suf_pccH (43 bp)'''<br>
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5'-CCATCACGGTCACGTACTGATACTAGTAGCGGCCGCTGCAGTC-3'<br>
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*Amplification of the cytochrome C (pccH) from ''Geobacter sulfurreducens KN400'' using the specific Gibson-Primer [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pccH_fwd pccH_fwd] and [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pccH_rev pccH_rev]:<br>
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 +
'''pccH_fwd (55 bp)'''<br>
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5'-ATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAAATGCAAAAAACAACTATTCTTTC-3'<br>
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'''pccH_rev (40 bp)'''<br>
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5'-TGCAGCGGCCGCTACTAGTATCAGTACGTGACCGTGATGG-3'<br>
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*[http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Gibson Gibson-Assembly] of the pSB1C3-Backbone and the cyctochrome c from ''Geobacter sulfurreducens'' containing also the RBS <bbpart>BBa_B0034</bbpart>.<br>
  
 
===Source===
 
===Source===
  
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Gibson
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*[http://www.ncbi.nlm.nih.gov/gene/?term=pcch%20geobacter%20sulfurreducens cytochrome c (''pccH'') from ''Geobacter sulfurreducens KN400'']
  
 
===References===
 
===References===
 +
 +
* Dantas JM, Tomaz DM, Morgado L, Salgueiro CA (2013) Functional characterization of PccH, a key cytochrome for electron transfer from electrodes to the bacterium Geobacter sulfurreducens. [http://ac.els-cdn.com/S0014579313005139/1-s2.0-S0014579313005139-main.pdf?_tid=34116a64-4b3d-11e4-89d7-00000aacb35e&acdnat=1412368876_a3d9578217a3772893836e278ab068e6 ''FEBS Letters 587(16):2662-8'']

Latest revision as of 20:38, 3 October 2014


Cytochrome c from Geobacter sulfurreducens


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 352
    Illegal SapI.rc site found at 433


Design Notes

  • Amplification of the pSB1C3-Backbone (BBa_J04450) using the specific Gibson-Primer [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_pre_pccH pSB1C3_pre_pccH] and [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_suf_pccH pSB1C3_suf_pccH]:
pSB1C3_pre_pccH (58 bp)

5'-GAAAGAATAGTTGTTTTTTGCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3'

pSB1C3_suf_pccH (43 bp)

5'-CCATCACGGTCACGTACTGATACTAGTAGCGGCCGCTGCAGTC-3'
  • Amplification of the cytochrome C (pccH) from Geobacter sulfurreducens KN400 using the specific Gibson-Primer [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pccH_fwd pccH_fwd] and [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pccH_rev pccH_rev]:
pccH_fwd (55 bp)

5'-ATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAAATGCAAAAAACAACTATTCTTTC-3'

pccH_rev (40 bp)

5'-TGCAGCGGCCGCTACTAGTATCAGTACGTGACCGTGATGG-3'
  • [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Gibson Gibson-Assembly] of the pSB1C3-Backbone and the cyctochrome c from Geobacter sulfurreducens containing also the RBS BBa_B0034.

Source

  • [http://www.ncbi.nlm.nih.gov/gene/?term=pcch%20geobacter%20sulfurreducens cytochrome c (pccH) from Geobacter sulfurreducens KN400]

References

  • Dantas JM, Tomaz DM, Morgado L, Salgueiro CA (2013) Functional characterization of PccH, a key cytochrome for electron transfer from electrodes to the bacterium Geobacter sulfurreducens. [http://ac.els-cdn.com/S0014579313005139/1-s2.0-S0014579313005139-main.pdf?_tid=34116a64-4b3d-11e4-89d7-00000aacb35e&acdnat=1412368876_a3d9578217a3772893836e278ab068e6 FEBS Letters 587(16):2662-8]