Difference between revisions of "Part:BBa K1379006:Design"
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<partinfo>BBa_K1379006 short</partinfo> | <partinfo>BBa_K1379006 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
− | + | This σ<sup>X</sup> Generator was constructed by restricting-ligating to downstream of [[Part:BBa_K880005|BBa_K880005]] first and then subsequently to [[Part:BBa_B0015|BBa_B0015]] in [[Part:pSB1C3|pSB1C3]]. | |
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===Source=== | ===Source=== | ||
− | + | iGEM 2014 Hong_Kong_HKUST Team has cloned σ<sup>X</sup> from <i>S. pneumoniae</i> strain NCTC7465. | |
===References=== | ===References=== | ||
+ | Luo P., & Morrison D. (2003).'' Transient Association of an Alternative Sigma Factor, ComX, with RNA Polymerase during the Period of Competence for Genetic Transformation in Streptococcus pneumoniae''. Journal of Bacteriology. doi:10.1128/JB.185.1.349-358.2003 | ||
+ | <br><br> | ||
+ | Piotrowski A., Luo P., & Morrison D. (2009). ''Competence for genetic transformation in Streptococcus pneumoniae: termination of activity of the alternative sigma factor ComX is independent of proteolysis of ComX and ComW.'' Journal of Bacteriology. doi:10.1128/JB.01750-08 | ||
+ | <br><br> |
Latest revision as of 21:23, 11 October 2014
S. pneumoniae σx Generator
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 359
Design Notes
This σX Generator was constructed by restricting-ligating to downstream of BBa_K880005 first and then subsequently to BBa_B0015 in pSB1C3.
Source
iGEM 2014 Hong_Kong_HKUST Team has cloned σX from S. pneumoniae strain NCTC7465.
References
Luo P., & Morrison D. (2003). Transient Association of an Alternative Sigma Factor, ComX, with RNA Polymerase during the Period of Competence for Genetic Transformation in Streptococcus pneumoniae. Journal of Bacteriology. doi:10.1128/JB.185.1.349-358.2003
Piotrowski A., Luo P., & Morrison D. (2009). Competence for genetic transformation in Streptococcus pneumoniae: termination of activity of the alternative sigma factor ComX is independent of proteolysis of ComX and ComW. Journal of Bacteriology. doi:10.1128/JB.01750-08