Difference between revisions of "Part:BBa K1114000"
 
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<html><head>
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__NOTOC__
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        <style>
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<partinfo>BBa_K1114000 short</partinfo>
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            div{
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                word-wrap:break-word;
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            }
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        </style>
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        <title class="name">BBa_E1010</title>
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        <link rel="shortcut icon" href="images/logo-Owl-Color_cropped.ico">
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        <link href="//netdna.bootstrapcdn.com/bootstrap/3.1.1/css/bootstrap.min.css" rel="stylesheet">
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        <link rel="stylesheet" type="text/css" media="print" href="//netdna.bootstrapcdn.com/bootstrap/3.1.1/css/bootstrap.min.css">
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        <meta http-equiv="Content-Type" content="text/html; charset=UTF-8">
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    <style type="text/css"></style></head>
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    <body class="container" id="main" style="padding-top: 10px;">
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        <h1><img style="width: 48px; height: 40px; margin-top:2px" src="images/logo-Owl-Color_cropped.png"><small> Automatically build Datasheets</small></h1>
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        <!--summary-->
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        <div class="well" id="basicInformation">
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            <h1 class="name">BBa_E1010</h1>
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<html>
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            <h3>Part Description</h3>  
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This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_J23100 with AB fusion sites. See the <a href="https://parts.igem.org/Part:BBa_J23100">BBa_J23100</a> page for full information on the part.
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    <div class="container">
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      <div class="row">
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          <div class="col-xs-6">
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            <h4>Summary</h4>
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                    <p id="summary">**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)</p>
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          </div>
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    <div class="col-xs-6">
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            <h4>Sequence</h4>
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                    <p id="sequence" style="width:80%;word-wrap: break-word;">atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagtt cgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgc tgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccg gactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgt tacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtc cggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaa atcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggt tcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagt acgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc</p>
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                    </div>
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                    </div>
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        <div class="row">
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          <div class="col-xs-6">
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            <h4>Pigeon Image</h4>
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            <div class="row-fluid span6" id="deviceImage"><img src="" class="img-polaroid" style="width:80%;"></div>
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                </div>
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              <div class="col-xs-6">
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            <h4>Plasmid Map</h4>
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            <div class="row-fluid span6" id="plasmidMap"><img src="" class="img-polaroid" style="width:80%;"></div>                 
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              </div>
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          </div>
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        <div class="row">
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            <div class="col-xs-6">
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                <h4> Part Type </h4>
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                <div id="partType">
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                                            Basic Part
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                                        </div>
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            </div>
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            <div class="col-xs-6">
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                <h4> Related Parts </h4>
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                <div id="relatedParts" style="width:80%"></div>
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            </div>
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        </div>
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        </div>
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        </div>
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        <div class="well" id="designerInfo">
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            <h3>Designer Information</h3>
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            <div class="container">
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                <div class="row">
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                    <div class="col-xs-4"><h4>Author(s)</h4><div id="authors">Drew Endy</div></div>
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                    <div class="col-xs-4"><h4>Data Collectors</h4><div id="dataCollection"></div></div>
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                    <div class="col-xs-4"><h4>Date</h4><div id="date" style="width:80%">2004-07-28</div></div>
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                </div>
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                <div class="row">
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                    <div class="col-xs-4"><h4>Affiliation</h4><div id="affiliation"></div></div>
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                    <div class="col-xs-4"><h4>Team</h4><div id="team"></div></div>
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                    <div class="col-xs-4"><h4>Contact</h4><div id="contact" style="width:80%"></div></div>
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                </div>
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            </div>
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        </div>
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<br><br>
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        <div class="well" id="designDetails">
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          <h3>Designer Details</h3>
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            <div class="container">
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                <div class="row">
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                    <div class="col-xs-3"><h4>Type</h4><div id="type"></div></div>
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                    <div class="col-xs-3"><h4>Vector</h4><div id="vector"></div></div>
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                    <div class="col-xs-3"><h4>Design Components</h4><div id="designComponents"></div></div>
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                    <div class="col-xs-3"><h4>Additional Comments</h4><div id="designComments" style="width:80%"></div></div>
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                </div>
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            </div>
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        </div>
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        <div class="well" id="assemblyInformation">
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          <h3>Assembly Information</h3>
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            <div class="container">
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                <div class="row">
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                    <div class="col-xs-3"><h4>Assembly Method(s)</h4><div id="assemblyMethod"></div></div>
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                    <div class="col-xs-3"><h4>Chassis</h4><div id="chassis"></div></div>
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                    <div class="col-xs-3"><h4>Assembly RFC</h4><div id="assemblyRFC"></div></div>
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                    <div class="col-xs-3"><h4>Strain</h4><div id="strain" style="width:80%"></div></div>
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                </div>
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                <div class="row">
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                    <div class="col-xs-3"><h4>Scars (y/n)</h4><div id="assemblyScars"></div></div>
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                    <div class="col-xs-3"><h4>Additional Comments</h4><div id="assemblyComments"></div></div>
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                    <div class="col-xs-3"><h4>Assembly Components</h4><div id="assemblyComponents" style="width:80%"></div></div>
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                </div>
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                <div class="row">
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                    <div class="col-xs-6"><h4>Assembly Graph</h4><div id="assemblyImage"><img src="" class="img-polaroid" style="width:80%;"></div></div>
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                </div>
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            </div>
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        </div>
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        <script src="//ajax.googleapis.com/ajax/libs/jquery/2.0.3/jquery.min.js"></script>
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        <script src="//netdna.bootstrapcdn.com/bootstrap/3.0.0/js/bootstrap.min.js"></script>
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        <script>
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            $.get("DataServlet", function(data) {
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                console.log(data);
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                // a function to check is an entire field is empty
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                var hasOwnProperty = Object.prototype.hasOwnProperty;
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                function isEmpty(obj) {
+
This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part.
 +
The fusion site letters refer to 4bp fusion sites: A        = GGAG;        B        = TACT; C        = AATG; D        = AGGT;        E        = GCTT; F        = CGCT; G        = TGCC; H        = ACTA.
  
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                // null and undefined are "empty"
+
<br><br>
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                if (obj == null) return true;
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                // Assume if it has a length property with a non-zero value
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Summary of modifications from original part:
−
                // that that property is correct.
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                //if (obj.length > 0)    return false;
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                if (obj.length === 0)  return true;
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                // Otherwise, does it have any properties of its own?
 
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                // Note that this doesn't handle
 
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                // toString and valueOf enumeration bugs in IE < 9
 
−
                for (var key in obj) {
 
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                    if (hasOwnProperty.call(obj, key)) return false;
 
−
                }
 
  
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                return true;
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Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See <a href="https://parts.igem.org/Part:BBa_K783055">BBa_K783055 </a>.
−
                }
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                //console.log(data);
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                $(".name").text(data.name);
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                $("#summary").text(data.summary);
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                $("#sequence").text(data.sequence);
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                $("#partType").text(data.partType);
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                $("#relatedParts").text(data.relatedParts);
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                $("#deviceImage img").attr('src', data.deviceImage);
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                $("#plasmidMap img").attr('src', data.plasmidMap);
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                //Contact Info
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                $("#authors").text(data.contactInformation.authors);
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                $("#team").text(data.contactInformation.team);
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                $("#dataCollection").text(data.contactInformation.dataCollection);
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                $("#affiliation").text(data.contactInformation.affiliation);
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                $("#date").text(data.contactInformation.date);
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                $("#contact").text(data.contactInformation.contact);
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                //Design Detail 
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                //console.log(data.designDetails);
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                $("#type").text(data.designDetails.type);
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                $("#vector").text(data.designDetails.vector);
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                $("#designComponents").text(data.designDetails.designComponents);
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                $("#designComments").text(data.designDetails.designComments);
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                //Assembly
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                $("#assemblyMethod").text(data.assemblyInformation.assemblyMethod);
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                $("#chassis").text(data.assemblyInformation.chassis);
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                $("#assemblyRFC").text(data.assemblyInformation.assemblyRFC);
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                $("#assemblyScars").text(data.assemblyInformation.assemblyScars);
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                $("#assemblyComponents").text(data.assemblyInformation.assemblyComponents);
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                $("#chasis").text(data.assemblyInformation.chasis);
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                $("#strain").text(data.assemblyInformation.strain);
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                $("#assemblyComments").text(data.assemblyInformation.assemblyComments);
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                $("#assemblyImage img").attr('src', data.assemblyImage);
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                //Assay Table 
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//                var rmap = data.restrictionMap;
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                if (!isEmpty(data.restrictionMap)) {
 
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                    $('#main').append('<div class="well"><h3>Restriction Map</h3><div class = "container"><div class = "row"><div class = "col-xs-3"><h4>Gel Buffer</h4><div>'+ data.restrictionMap.GelBuffer +'</div></div><div class = "col-xs-3"><h4>DNA Quality</h4><div>'+data.restrictionMap.DNAQuantity +'</div></div><div class = "col-xs-3"><h4>Percent Agar</h4><div>'+data.restrictionMap.Agar +'</div></div><div class = "col-xs-3"><h4>Enzymes</h4><div style = "width:80%">'+data.restrictionMap.Enzymes
 
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                            +'</div></div></div><div class = "row"><div class = "col-xs-3"><h4>Ladder</h4><div>'+ data.restrictionMap.Ladder +'</div></div><div class = "col-xs-3"><h4>Digest Buffer</h4><div>'+data.restrictionMap.DigestBuffer +'</div></div><div class = "col-xs-3"><h4>Staining Dye</h4><div>'+data.restrictionMap.StainingDye +'</div></div><div class = "col-xs-3"><h4>Digest Time</h4><div style = "width:80%">'+data.restrictionMap.DigestTime
 
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                            + '</div></div></div><div class = "row"><div class = "col-xs-3"><h4>Voltage</h4><div>'+ data.restrictionMap.Voltage +'</div></div><div class = "col-xs-3"><h4>Gel Image</h4><div><img src="'+data.restrictionMap.Image+'" class="img-polaroid" style = "width:80%;"/>'+ '</div></div><div class = "col-xs-3"><h4>Time</h4><div>'+data.restrictionMap.Time +'</div></div><div class = "col-xs-3"><h4>Caption for Image</h4><div style = "width:80%">'+data.restrictionMap.Caption
 
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                            + '</div></div></div>'
 
−
        );
 
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                }
 
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                if (!isEmpty(data.functionalityAssays)) {
 
−
                    $('#main').append('<div class="well"><h3>Flow Cytometry Experiment</h3><div class = "container"><div class = "row"><div class = "col-xs-3"><h4>Purpose</h4><div>'+ data.functionalityAssays.Purpose +'</div></div><div class = "col-xs-3"><h4>Type</h4><div>'+data.functionalityAssays.ExperimentType +'</div></div><div class = "col-xs-3"><h4>Location</h4><div>'+data.functionalityAssays.Location +'</div></div><div class = "col-xs-3"><h4>FCS Format</h4><div style = "width:80%">'+data.functionalityAssays.FCSFormat
 
−
                            +'</div></div></div><div class = "row"><div class = "col-xs-3"><h4>Machine Name</h4><div>'+ data.functionalityAssays.MachineName +'</div></div><div class = "col-xs-3"><h4>Protocol Details</h4><div>'+ data.functionalityAssays.FlowProtocolDetails +'</div></div><div class = "col-xs-3"><h4>Link to Protocol</h4><div>'+ data.functionalityAssays.FlowProtocolLink +'</div></div><div class = "col-xs-3"><h4>Laser (filters)</h4><div style = "width:80%">'+data.functionalityAssays.LasersFilters
 
−
                            + '</div></div></div><div class = "row"><div class = "col-xs-3"><h4>Transfer Curve Graph</h4><div><img src="'+data.functionalityAssays.TranserCurve+'" class="img-polaroid" style = "width:80%;"/>'+ '</div></div><div class = "col-xs-3"><h4>Transfer Curve Captions</h4><div>'+ data.functionalityAssays.TranserCurveCaption +'</div></div><div class = "col-xs-3"><h4>Time Series Graph</h4><div><img src="'+data.functionalityAssays.TimeSeriesGraph+'" class="img-polaroid" style = "width:80%;"/></div></div><div class = "col-xs-3"><h4>Time Series Graph Caption</h4><div style = "width:80%">'+data.functionalityAssays.TimeSeriesGraphCaption
 
−
                            + '</div></div></div></div><h4 style = "margin-top:25px;">Pre-Induction Growth Conditions</h4><div class = "container"><div class = "row"><div class = "col-xs-4"><h4>Media Type</h4><div>'+ data.pre.PreMedia +'</div></div><div class = "col-xs-4"><h4>Vessel</h4><div>'+data.pre.PreVessel +'</div></div><div class = "col-xs-4"><h4>Volume</h4><div>'+data.pre.PreVolume
 
−
                            +'</div></div></div><div class = "row"><div class = "col-xs-4"><h4>Incubation Time (min)</h4><div>' + data.pre.PreIncubationTime+'</div></div><div class = "col-xs-4"><h4>Growth Phase</h4><div>'+ data.pre.PreGrowthPhase
 
−
                            +'</div></div></div></div><h4 style = "margin-top:25px;">Post-Induction Growth Conditions</h4><div class = "container"><div class = "row"><div class = "col-xs-4"><h4>Media Type</h4><div>'+ data.post.IndMedia +'</div></div><div class = "col-xs-4"><h4>Vessel</h4><div>'+data.post.IndVessel +'</div></div><div class = "col-xs-4"><h4>Volume</h4><div>'+data.post.IndVolume
 
−
                            +'</div></div></div><div class = "row"><div class = "col-xs-4"><h4>Incubation Time (min)</h4><div>' + data.post.IndIncubationTime+'</div></div><div class = "col-xs-4"><h4>Growth Phase</h4><div>'+ data.post.IndGrowthPhase+'</div></div><div class = "col-xs-4"><h4>Inducer</h4><div>'+data.post.Inducer
 
−
                            +'</div></div></div><div class = "row"><div class = "col-xs-4"><h4>Inducer Concentration</h4><div>' + data.post.InducerConcentration+'</div></div></div>')
 
−
                           
 
−
               
 
−
               
 
−
                /*
 
−
                    '</tbody></table><h5>Pre-Induction Growth Conditions</h5><table><tbody>'+
 
−
                    '<tr><th align="left">Media Type</th><td>' + data.pre.PreMedia + '</td></tr>' +
 
−
                    '<tr><th align="left">Vessel</th><td>' + data.pre.PreVessel + '</td></tr>' +
 
−
                    '<tr><th align="left">Volume</th><td>' + data.pre.PreVolume + '</td></tr>' +
 
−
                    '<tr><th align="left">Incubation Time (min)</th><td>' + data.pre.PreIncubationTime + '</td></tr>' +
 
−
                    '<tr><th align="left">Growth Phase</th><td>' + data.pre.PreGrowthPhase + '</td></tr>' +
 
−
                    '</tbody></table><h5>Pre-Induction Growth Conditions</h5><table><tbody>'+
 
−
                    '<tr><th align="left">Media Type</th><td>' + data.post.IndMedia + '</td></tr>' +
 
−
                    '<tr><th align="left">Vessel</th><td>' + data.post.IndVessel + '</td></tr>' +
 
−
                    '<tr><th align="left">Volume</th><td>' + data.post.IndVolume + '</td></tr>' +
 
−
                    '<tr><th align="left">Incubation Time (min)</th><td>' + data.post.IndIncubationTime + '</td></tr>' +
 
−
                    '<tr><th align="left">Growth Phase</th><td>' + data.post.IndGrowthPhase + '</td></tr>' +
 
−
                    '<tr><th align="left">Inducer</th><td>' + data.post.Inducer + '</td></tr>' +
 
−
                    '<tr><th align="left">Inducer Concentrations</th><td>' + data.post.InducerConcentration + '</td></tr>' +
 
−
                    '</div></div>');*/
 
−
       
 
−
                }
 
−
   
 
−
                if (!isEmpty(data.otherAssay)) {
 
−
                    $('#main').append('<div class="well"><h3>Custom Assay</h3><div class = "container"><div class = "row"><div class = "col-xs-3"><h4>Assay Name</h4><div>'+ data.otherAssay.AssayName +'</div></div><div class = "col-xs-3"><h4>Protocol Details</h4><div>'+data.otherAssay.ProtocolDetails +'</div></div><div class = "col-xs-3"><h4>Assay Type</h4><div>'+data.otherAssay.AssayPurpose +'</div></div><div class = "col-xs-3"><h4>Link to Protocol</h4><div style = "width:80%">'+data.otherAssay.ProtocolLink
 
−
                            +'</div></div></div><div class = "row"><div class = "col-xs-3"><h4>Image</h4><div><img src="'+data.otherAssay.AssayImage+'" class="img-polaroid" style = "width:80%;"/></div></div><div class = "col-xs-3"><h4>Image Caption</h4><div>'+ data.otherAssay.AssayImageCaption +'</div></div><div class = "col-xs-3"><h4>Link to Additional Data</h4><div>'+ data.otherAssay.AssayDataLink +'</div></div><div class = "col-xs-3"><h4>Additional Data Details</h4><div style = "width:80%">'+ data.otherAssay.AssayDataDetails
 
−
                            + '</div></div></div><div class = "row"><div class = "col-xs-3"><h4>Parameter 1</h4><div>' + data.otherAssay.Parameter1+'</div></div><div class = "col-xs-3"><h4>Value 1</h4><div>'+ data.otherAssay.Value1 +'</div></div><div class = "col-xs-3"><h4>Parameter 2</h4><div>'+ data.otherAssay.Parameter2 +'</div></div><div class = "col-xs-3"><h4>Value 2</h4><div style = "width:80%">'+data.otherAssay.Value2
 
−
                            + '</div></div></div><div class = "row"><div class = "col-xs-3"><h4>Parameter 3</h4><div>' + data.otherAssay.Parameter3+'</div></div><div class = "col-xs-3"><h4>Value 3</h4><div>'+ data.otherAssay.Value3 +'</div></div><div class = "col-xs-3"><h4>Additional Comments</h4><div>'+ data.otherAssay.comments +'</div></div>'
 
−
                           
 
−
               
 
−
               
 
−
               
 
−
                /*'<div class="well"><h3>Custom Assay</h3><div class="row-fluid"> <table><col width="250"><col width="250"><tbody>'+
 
−
                    '<tr><th align="left">Assay Name</th><td>' + data.otherAssay.AssayName + '</td> <th align="left">Protocol Details</th><td>' + data.otherAssay.ProtocolDetails + '</td></tr>' +
 
−
                    '<tr><th align="left">Assay Type</th><td>' + data.otherAssay.AssayPurpose + '</td> <th align="left">Link to Protocol</th><td>' + data.otherAssay.ProtocolLink + '</td></tr>' +
 
−
                    '<tr><th align="left">Link to Image</th><td>' + data.otherAssay.AssayImage + '</td> <th align="left">Image Caption</th><td>' + data.otherAssay.AssayImageCaption + '</td></tr>' +
 
−
                    '<tr><th align="left">Link to Additional Data</th><td>' + data.otherAssay.AssayDataLink + '</td> <th align="left">Additional Data Details</th><td>' + data.otherAssay.AssayDataDetails + '</td></tr>' +
 
−
                    '<tr><th align="left">Parameter 1</th><td>' + data.otherAssay.Parameter1 + '</td> <th align="left">Value 1</th><td>' + data.otherAssay.Value1 + '</td></tr>' +
 
−
                    '<tr><th align="left">Parameter 2</th><td>' + data.otherAssay.Parameter2 + '</td> <th align="left">Value 2</th><td>' + data.otherAssay.Value2 + '</td></tr>' +
 
−
                    '<tr><th align="left">Parameter 3</th><td>' + data.otherAssay.Parameter3 + '</td> <th align="left">Value 3</th><td>' + data.otherAssay.Value3 + '</td></tr>' +
 
−
                    '</tbody></table></div></div>'*/);
 
−
                }             
 
−
            });
 
−
        </script>
 
−
       
 
−
        <!--<script>$('div.well:empty').hide(); </script>-->
 
−
       
 
−
   
 
  
 +
</html>
  
−
</body></html>
+
 
 +
<partinfo>BBa_K1114000 SequenceAndFeatures</partinfo>
 +
 
 +
===Source===
 +
 
 +
 
 +
iGEM Distribution Kit
 +
 
 +
 
 +
===Functional Parameters===
 +
<partinfo>BBa_K1114001 parameters</partinfo>

Latest revision as of 01:26, 30 April 2014

The MoClo format of BBa_J23100 with AB fusion sites.

This is the MoClo formatted part of BBa_J23100 with AB fusion sites. See the BBa_J23100 page for full information on the part.

This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.

Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 .



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Source

iGEM Distribution Kit


Functional Parameters