Difference between revisions of "Part:BBa K1031917"

Latest revision as of 16:20, 28 October 2013

J23117-XylS-Terminator

For detailed information concerning XylS, please visit 2013 Peking iGEM Biosensor XylS

Characterization

In order to fine-tune the performance of XylS biosensor, we constructed a library of Pc constitutive promoters at different intensity. BBa_K1031917 is composed of three elements, the constitutive Pc promoter J23117, coding sequence of XylS and terminator B0015. (Fig 1)

Fig 1 Construction of biosensor circuit Pm/J23117-XylS. Orange arrowheads represent promoters. RBSs(Ribosome Binding Sites) are shown as green ovals and squares in dark red stand for B0015 terminator.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 94
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 816
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 279

Data shown

Dose response curve of biosensor circuit Pm/J23117-XylS when exposed to 4 typical inducers at different concentration. This biosensor performs well.(Fig 2)

Fig 2 4 inducers are shown in different colors. Horizontal axis stands for different concentration of BzO, 2-,3-,4-BzO. The test was following Test Protocol 1. Vertical axis represents the induction ratio calculated from fluorescence intensity.

@@ Line 16: / Line 16: @@
 <img src="https://static.igem.org/mediawiki/2013/thumb/d/d0/Peking2013_part_117-XylS_construction.png/800px-Peking2013_part_117-XylS_construction.png"
 style="width:600px; margin-left:170px" />
-<p style="text-align:center"><b>Fig 1</b> Construction of biosensor circuit <i>Pm</i>/J23117-XylS. Orange arrowheads represent promoters. RBSs are shown as green ovals and squares in dark red stand for B0015 terminator.
+<p style="text-align:center"><b>Fig 1</b> Construction of biosensor circuit <i>Pm</i>/J23117-XylS. Orange arrowheads represent promoters. RBSs(Ribosome Binding Sites) are shown as green ovals and squares in dark red stand for B0015 terminator.
 </html>
@@ Line 31: / Line 31: @@
 == '''Data shown''' ==
-Induction ratio of XylS biosensor library from ON/OFF test that utilizes different ''Pc'' promoters to control the expression of XylS. The dashed box refers to data for ''Pm''/J23117-XylS biosensor circuit ('''Fig 2''')
+Dose response curve of biosensor circuit ''Pm''/J23117-XylS when exposed to 4 typical inducers at different concentration. This biosensor performs well.('''Fig 2''')
 <html>
-<img src="" /
+<img src="https://static.igem.org/mediawiki/2013/thumb/8/81/Peking2013_part_117XylS_Typical_Inducers.jpg/800px-Peking2013_part_117XylS_Typical_Inducers.jpg" /
 style="width:600px; margin-left:170px">
-<p style="text-align:center"><b>Fig 2</b> Horizontal axis stands for different XylS biosensor with <i>Pc</i> promoters of different strength. The expression strength of these constitutive promoters, J23113, J23109, J23114, J23105, J23106 is 21, 106, 256, 623, and 1185, respectively, according to the Parts' registry. Four kinds of aromatics, namely BzO, 2-MxeBzO, 3-MeBzO and 4-MeBzO, shown with different color intensities, were tested following <a href="http://2013.igem.org/Team:Peking/Team/Notebook/Protocols">Test Protocol 1</a>. Vertical axis represents the ON/OFF induction ratio. The dashed box refers to performance for <i>Pm</i>/J23117-XylS biosensor circuit.
+<p style="text-align:center"><b>Fig 2</b> 4 inducers are shown in different colors. Horizontal axis stands for different concentration of BzO, 2-,3-,4-BzO. The test was following <a href="http://2013.igem.org/Team:Peking/Team/Notebook/Protocols">Test Protocol 1</a>. Vertical axis represents the induction ratio calculated from fluorescence intensity.
 </html>