Difference between revisions of "Part:BBa K1031917"

 
 
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<partinfo>BBa_K1031917 short</partinfo>
 
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<p>For detailed information concerning XylS, please visit <a href="http://2013.igem.org/Team:Peking/Project/BioSensors/XylS">2013 Peking iGEM Biosensor XylS</a></p>
  
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117-XylS-TT
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<img src="https://static.igem.org/mediawiki/igem.org/c/c9/Peking_Logo.jpg" style="width:960px;"/>
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===Usage and Biology===
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== '''Characterization''' ==
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In order to fine-tune the performance of XylS biosensor, we constructed a library of ''Pc'' constitutive promoters at different intensity. BBa_K1031917 is composed of three elements, the constitutive ''Pc'' promoter <html><a href="https://parts.igem.org/Part:BBa_J23105">J23117</a></html>, coding sequence of XylS and terminator <html><a href="https://parts.igem.org/Part:BBa_B0015">B0015</a></html>. ('''Fig 1''')
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<img src="https://static.igem.org/mediawiki/2013/thumb/d/d0/Peking2013_part_117-XylS_construction.png/800px-Peking2013_part_117-XylS_construction.png"
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style="width:600px; margin-left:170px" />
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<p style="text-align:center"><b>Fig 1</b> Construction of biosensor circuit <i>Pm</i>/J23117-XylS. Orange arrowheads represent promoters. RBSs(Ribosome Binding Sites) are shown as green ovals and squares in dark red stand for B0015 terminator.
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== '''Sequence and Features''' ==
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1031917 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1031917 SequenceAndFeatures</partinfo>
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== '''Data shown''' ==
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Dose response curve of biosensor circuit ''Pm''/J23117-XylS when exposed to 4 typical inducers at different concentration. This biosensor performs well.('''Fig 2''')
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<img src="https://static.igem.org/mediawiki/2013/thumb/8/81/Peking2013_part_117XylS_Typical_Inducers.jpg/800px-Peking2013_part_117XylS_Typical_Inducers.jpg" /
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<p style="text-align:center"><b>Fig 2</b> 4 inducers are shown in different colors. Horizontal axis stands for different concentration of BzO, 2-,3-,4-BzO. The test was following <a href="http://2013.igem.org/Team:Peking/Team/Notebook/Protocols">Test Protocol 1</a>. Vertical axis represents the induction ratio calculated from fluorescence intensity.
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Latest revision as of 16:20, 28 October 2013

J23117-XylS-Terminator

For detailed information concerning XylS, please visit 2013 Peking iGEM Biosensor XylS


Characterization

In order to fine-tune the performance of XylS biosensor, we constructed a library of Pc constitutive promoters at different intensity. BBa_K1031917 is composed of three elements, the constitutive Pc promoter J23117, coding sequence of XylS and terminator B0015. (Fig 1)

Fig 1 Construction of biosensor circuit Pm/J23117-XylS. Orange arrowheads represent promoters. RBSs(Ribosome Binding Sites) are shown as green ovals and squares in dark red stand for B0015 terminator.


Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 94
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 816
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 279


Data shown

Dose response curve of biosensor circuit Pm/J23117-XylS when exposed to 4 typical inducers at different concentration. This biosensor performs well.(Fig 2)

Fig 2 4 inducers are shown in different colors. Horizontal axis stands for different concentration of BzO, 2-,3-,4-BzO. The test was following Test Protocol 1. Vertical axis represents the induction ratio calculated from fluorescence intensity.