Difference between revisions of "Part:BBa K1150043"
 
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===Functional Parameters===
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==Functional Parameters: Austin_UTexas==
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<partinfo>BBa_K1150043 parameters</partinfo>
 
<partinfo>BBa_K1150043 parameters</partinfo>
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<h3><center>Burden Imposed by this Part:</center></h3>
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<center><img src = "https://static.igem.org/mediawiki/parts/f/fa/T--Austin_Utexas--no_burden_icon.png" style = "width:160px;height:120px"></center>
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<figcaption><center><b>Burden Value: -1.2 ± 2.6% </b></center></figcaption>
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<p> Burden is the percent reduction in the growth rate of <i>E. coli</i> cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the
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<a href="https://parts.igem.org/Part:BBa_K3174002">BBa_K3174002</a> - <a href="https://parts.igem.org/Part:BBa_K3174007">BBa_K3174007</a> pages for more information on the methods, an explanation of the sources of burden,  and other conclusions from a large-scale measurement project conducted by the <a href="http://2019.igem.org/Team:Austin_UTexas">2019 Austin_UTexas team</a>.</p>
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<p>This functional parameter was added by the <a href="https://2020.igem.org/Team:Austin_UTexas/Contribution">2020 Austin_UTexas team.</a></p>
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Latest revision as of 04:26, 25 August 2020

uniCAS RNAimer multiple targets to VEGF 2 and VEGF 3

pH1:tracrRNA pU6:DR_dummy_DR
Function two small RNAs giving rise to

specific binding of dCas9 to two VEGF loci

Use in Mammalian cells
RFC standard RFC 25
Backbone pSB1C3
Organism Streptococcus pyogenes
Source Feng Zhang, Addgene
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]



Usage and Biology

By combining two single loci via the idempotent cloning strategy two loci were connected into one RNA plasmid. This enables multiple targeting of two loci with the [http://2013.igem.org/Team:Freiburg/Project/effector dCas9 derivates] at once. The basis of the device is the RNAimer.
Here, we combined BBa_K1150037 and BBa_K1150038.


Sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 224
    Illegal BglII site found at 1061
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 216
    Illegal BsaI.rc site found at 1053


Functional Parameters: Austin_UTexas

BBa_K1150043 parameters

Burden Imposed by this Part:

Burden Value: -1.2 ± 2.6%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.