Difference between revisions of "Part:BBa K1065307"
| Line 4: | Line 4: | ||
This is the intermediate part to build a blue light inducible genetic circuit. The part produces YF1 and FixJ under the control of a strong promoter of the Anderson family <partinfo>BBa_J23100</partinfo>. Two terminators are placed after FixJ. | This is the intermediate part to build a blue light inducible genetic circuit. The part produces YF1 and FixJ under the control of a strong promoter of the Anderson family <partinfo>BBa_J23100</partinfo>. Two terminators are placed after FixJ. | ||
| − | Note | + | Note. this part was not yet submitted to the registry but we are going to submitt it soon. Meanwhile, if you are interested in this part please contact iGEM UniTN Trento 2013. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 09:38, 3 October 2013
J23100+YF1+FixJ+terminator
This is the intermediate part to build a blue light inducible genetic circuit. The part produces YF1 and FixJ under the control of a strong promoter of the Anderson family BBa_J23100. Two terminators are placed after FixJ.
Note. this part was not yet submitted to the registry but we are going to submitt it soon. Meanwhile, if you are interested in this part please contact iGEM UniTN Trento 2013.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 605
Illegal NgoMIV site found at 677
Illegal NgoMIV site found at 767
Illegal NgoMIV site found at 785
Illegal NgoMIV site found at 1297
Illegal NgoMIV site found at 1590
Illegal NgoMIV site found at 1684
Illegal AgeI site found at 319
Illegal AgeI site found at 1465 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1354
Illegal BsaI.rc site found at 218