Difference between revisions of "Part:BBa K1073011:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K1073011=== | ===Applications of BBa_K1073011=== | ||
| − | iGEM Team Braunschweig 2013 used this device for the contruction of <partinfo>BBa_K1073035</partinfo>. It was used in order to induce growth in the presence of RhlR and N- | + | iGEM Team Braunschweig 2013 used this device for the contruction of <partinfo>BBa_K1073035</partinfo>. It was used in order to induce growth in the presence of RhlR and N-butyryl-HSL in ampicillin containing media. |
===User Reviews=== | ===User Reviews=== | ||
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<I>iGEM Team Braunschweig 2013</I> | <I>iGEM Team Braunschweig 2013</I> | ||
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| − | This device was successfully employed in <partinfo>BBa_K1073035</partinfo> to mediate ampicillin resistance in the presence of RhlR and N- | + | This device was successfully employed in <partinfo>BBa_K1073035</partinfo> to mediate ampicillin resistance in the presence of RhlR and N-butyryl-HSL. Due to a low level leakiness of the promoter and the bacteriostatic properties of ampicillin, the cells will start to grow in batch cultivations when a low level of ampicillin is reached even whithout N-butyryl-HSL.Uninduced activity of the promoter differs depending on strain and media used. |
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Latest revision as of 08:57, 17 October 2013
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1073011
iGEM Team Braunschweig 2013 used this device for the contruction of BBa_K1073035. It was used in order to induce growth in the presence of RhlR and N-butyryl-HSL in ampicillin containing media.
User Reviews
UNIQ7b035021fadb0118-partinfo-00000001-QINU
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iGEM Team Braunschweig 2013 |
This device was successfully employed in BBa_K1073035 to mediate ampicillin resistance in the presence of RhlR and N-butyryl-HSL. Due to a low level leakiness of the promoter and the bacteriostatic properties of ampicillin, the cells will start to grow in batch cultivations when a low level of ampicillin is reached even whithout N-butyryl-HSL.Uninduced activity of the promoter differs depending on strain and media used. UNIQ7b035021fadb0118-partinfo-00000004-QINU |