Difference between revisions of "Part:BBa K1065307"
 
(5 intermediate revisions by 3 users not shown)
Line 2: Line 2:
 
<partinfo>BBa_K1065307 short</partinfo>
 
<partinfo>BBa_K1065307 short</partinfo>
  
−
This is the intermediate part to build a blue light inducible genetic circuit <partinfo>BBa_K1065307</partinfo> in which it was added the terminator <partinfo>BBa_B0015</partinfo> and was moved in pSB1C3. The part produces YF1 and FixJ under tho control of a strong promoter of the Anderson family.
+
This is the intermediate part to build a blue light inducible genetic circuit. The part produces YF1 and FixJ under the control of a strong promoter of the Anderson family <partinfo>BBa_J23100</partinfo>. Two terminators are placed after FixJ.
 +
 
 +
Note. this part was not yet submitted to the registry but we are going to submitt it soon. Meanwhile, if you are interested in this part please contact iGEM UniTN Trento 2013.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 09:38, 3 October 2013

J23100+YF1+FixJ+terminator

This is the intermediate part to build a blue light inducible genetic circuit. The part produces YF1 and FixJ under the control of a strong promoter of the Anderson family BBa_J23100. Two terminators are placed after FixJ.

Note. this part was not yet submitted to the registry but we are going to submitt it soon. Meanwhile, if you are interested in this part please contact iGEM UniTN Trento 2013.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 605
    Illegal NgoMIV site found at 677
    Illegal NgoMIV site found at 767
    Illegal NgoMIV site found at 785
    Illegal NgoMIV site found at 1297
    Illegal NgoMIV site found at 1590
    Illegal NgoMIV site found at 1684
    Illegal AgeI site found at 319
    Illegal AgeI site found at 1465
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1354
    Illegal BsaI.rc site found at 218