Difference between revisions of "Part:BBa K1073026"
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<partinfo>BBa_K1073026 short</partinfo> | <partinfo>BBa_K1073026 short</partinfo> | ||
| − | + | This device is an enhanced construct of 2012 Uppsala's <partinfo>BBa_K592011</partinfo>. It consists of a strong promoter (<partinfo>BBa_J23100</partinfo>) combined with RBS (<partinfo>BBa_B0032</partinfo>) and the encoding region of the chromoprotein cjBlue (<partinfo>BBa_K592011</partinfo>). This results in a constitutively high expression of cjBlue. | |
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | During cultivation the chromoprotein cjBlue is expressed and stored | + | During cultivation, the chromoprotein cjBlue is expressed and stored intracellularly. The greenish color of the protein can be seen by the naked eye, thus the chromoprotein can be used as a reporter to distinguish cells easily. |
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Latest revision as of 18:01, 4 October 2013
Constitutively expressed chromoprotein cjBlue
This device is an enhanced construct of 2012 Uppsala's BBa_K592011. It consists of a strong promoter (BBa_J23100) combined with RBS (BBa_B0032) and the encoding region of the chromoprotein cjBlue (BBa_K592011). This results in a constitutively high expression of cjBlue.
Usage and Biology
During cultivation, the chromoprotein cjBlue is expressed and stored intracellularly. The greenish color of the protein can be seen by the naked eye, thus the chromoprotein can be used as a reporter to distinguish cells easily.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]