Difference between revisions of "Part:BBa K1073011:Experience"

Latest revision as of 08:57, 17 October 2013

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1073011

iGEM Team Braunschweig 2013 used this device for the contruction of BBa_K1073035. It was used in order to induce growth in the presence of RhlR and N-butyryl-HSL in ampicillin containing media.

User Reviews

UNIQ66e8057044013f6b-partinfo-00000001-QINU

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iGEM Team Braunschweig 2013

This device was successfully employed in BBa_K1073035 to mediate ampicillin resistance in the presence of RhlR and N-butyryl-HSL. Due to a low level leakiness of the promoter and the bacteriostatic properties of ampicillin, the cells will start to grow in batch cultivations when a low level of ampicillin is reached even whithout N-butyryl-HSL.Uninduced activity of the promoter differs depending on strain and media used. UNIQ66e8057044013f6b-partinfo-00000004-QINU

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 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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 ===Applications of BBa_K1073011===
-iGEM Team Braunschweig 2013 used this device for the contruction of <partinfo>BBa_K1073035</partinfo>. It was used in order to induce growth in the presence of RhlR and N-buturyl-HSL in ampicillin containing media.
+iGEM Team Braunschweig 2013 used this device for the contruction of <partinfo>BBa_K1073035</partinfo>. It was used in order to induce growth in the presence of RhlR and N-butyryl-HSL in ampicillin containing media.
 ===User Reviews===
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-<partinfo>BBa_K1073011 </partinfo>
+<partinfo>BBa_K1073011 AddReview 4 </partinfo>
 <I>iGEM Team Braunschweig 2013</I>
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-This device was successfully employed in BBa_K1073035 to mediate ampicillin resistance in the presence of RhlR and N-buturyl-HSL. Due to the low level leakiness of the promoter cells also grow when N-buturyl-HSL is not present. However, growth is much higher when both RhlR and N-buturyl-HSL are present.
+This device was successfully employed in <partinfo>BBa_K1073035</partinfo> to mediate ampicillin resistance in the presence of RhlR and N-butyryl-HSL. Due to a low level leakiness of the promoter and the bacteriostatic properties of ampicillin, the cells will start to grow in batch cultivations when a low level of ampicillin is reached even whithout N-butyryl-HSL.Uninduced activity of the promoter differs depending on strain and media used.
-|};
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 <!-- DON'T DELETE --><partinfo>BBa_K1073011 EndReviews</partinfo>