Difference between revisions of "Part:BBa K1073011:Experience"

Latest revision as of 08:57, 17 October 2013

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Please enter how you used this part and how it worked out.

Applications of BBa_K1073011

iGEM Team Braunschweig 2013 used this device for the contruction of BBa_K1073035. It was used in order to induce growth in the presence of RhlR and N-butyryl-HSL in ampicillin containing media.

User Reviews

UNIQa65486257a3296aa-partinfo-00000001-QINU

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iGEM Team Braunschweig 2013

This device was successfully employed in BBa_K1073035 to mediate ampicillin resistance in the presence of RhlR and N-butyryl-HSL. Due to a low level leakiness of the promoter and the bacteriostatic properties of ampicillin, the cells will start to grow in batch cultivations when a low level of ampicillin is reached even whithout N-butyryl-HSL.Uninduced activity of the promoter differs depending on strain and media used. UNIQa65486257a3296aa-partinfo-00000004-QINU

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 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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 ===Applications of BBa_K1073011===
-iGEM Team Braunschweig 2013 used this device for the contruction of <partinfo>BBa_K1073035</partinfo>. It was used in order to induce growth in the presence of RhlR and N-buturyl-HSL in ampicillin containing media.
+iGEM Team Braunschweig 2013 used this device for the contruction of <partinfo>BBa_K1073035</partinfo>. It was used in order to induce growth in the presence of RhlR and N-butyryl-HSL in ampicillin containing media.
 ===User Reviews===
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-<partinfo>BBa_K1073011 </partinfo>
+<partinfo>BBa_K1073011 AddReview 4 </partinfo>
 <I>iGEM Team Braunschweig 2013</I>
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-This device was successfully employed in BBa_K1073035 to mediate ampicillin resistance in the presence of RhlR and N-buturyl-HSL. Due to the low level leakiness of the promoter cells also grow when N-buturyl-HSL is not present. However, growth is much higher when both RhlR and N-buturyl-HSL are present.
+This device was successfully employed in <partinfo>BBa_K1073035</partinfo> to mediate ampicillin resistance in the presence of RhlR and N-butyryl-HSL. Due to a low level leakiness of the promoter and the bacteriostatic properties of ampicillin, the cells will start to grow in batch cultivations when a low level of ampicillin is reached even whithout N-butyryl-HSL.Uninduced activity of the promoter differs depending on strain and media used.
-|};
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 <!-- DON'T DELETE --><partinfo>BBa_K1073011 EndReviews</partinfo>