Difference between revisions of "Part:BBa K1033282"
(→‎Team KUAS_Korea 2017: characterization)
 
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<partinfo>BBa_K1033282 short</partinfo>
 
<partinfo>BBa_K1033282 short</partinfo>
  
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This is a blue/purple chromoprotein (amilCP: [[part:BBa_K592009|BBa_K592009]]) with the RBS B0034 ([[part:BBa_B0034|BBa_B0034]]) and a strong promoter (CP29: [[part:BBa_K1033222|BBa_K1033222]]). The promoter is supposed to work in E. coli as well as Lactobacillus.  
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This is a blue/purple chromoprotein (amilCP: [[part:BBa_K592009|BBa_K592009]]) with the RBS B0034 ([[part:BBa_B0034|BBa_B0034]]) and a strong promoter (CP29: [[part:BBa_K1033222|BBa_K1033222]]). The construct has been shown to work in E. coli and should work in Lactobacillus.
  
 
(Construct name: pSB1C3-CP29-B0034-amilCP)
 
(Construct name: pSB1C3-CP29-B0034-amilCP)
  
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More coming soon...
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See http://2013.igem.org/Team:Uppsala/reporter-genes for more information.
  
 
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<partinfo>BBa_K1033282 parameters</partinfo>
 
<partinfo>BBa_K1033282 parameters</partinfo>
 
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==Team KUAS_Korea 2017: characterization==
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We tested the functionality of K1033222 as a constitutive promoter and expression of amilCP chromoprotein in the <i>Escherichia coli</i> DH5alpha.
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DH5alpha containing the control(pSB1C3) and BBa_K1033282, respectively, were cultured in LB medium containing chloramphenicol.
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Absorbances were measured every 30 minutes using BioRad SmartSpecPlus at 588 nm and 800 nm.
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[[Image:T--KUAS Korea--amilCP.png|500px|center|]]
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<br>
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[[File:OD588-time-re-KUAS-Korea.png|500px|center|]]
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<br>
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[[File:OD588-800-ratio-KUAS-Korea.png|500px|center|]]
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[[File:--File-OD588-800-KUAS-Korea-500px-center--.png|center|]]
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The first photo is a transformation of BBa_K1033282 to DH5alpha. This was then grown in LB medium supplemented with chloramphenicol at 37 degrees Celsius. The first graph shows changes in OD588 over time, the second shows changes in OD588 / OD800 ratio with time, and the third shows OD588 according to OD800.

Latest revision as of 03:29, 28 October 2017

amilCP blue chromoprotein with strong promoter

This is a blue/purple chromoprotein (amilCP: BBa_K592009) with the RBS B0034 (BBa_B0034) and a strong promoter (CP29: BBa_K1033222). The construct has been shown to work in E. coli and should work in Lactobacillus.

(Construct name: pSB1C3-CP29-B0034-amilCP)

See http://2013.igem.org/Team:Uppsala/reporter-genes for more information.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Team KUAS_Korea 2017: characterization

We tested the functionality of K1033222 as a constitutive promoter and expression of amilCP chromoprotein in the Escherichia coli DH5alpha.

DH5alpha containing the control(pSB1C3) and BBa_K1033282, respectively, were cultured in LB medium containing chloramphenicol.

Absorbances were measured every 30 minutes using BioRad SmartSpecPlus at 588 nm and 800 nm.


T--KUAS Korea--amilCP.png


OD588-time-re-KUAS-Korea.png


OD588-800-ratio-KUAS-Korea.png


--File-OD588-800-KUAS-Korea-500px-center--.png


The first photo is a transformation of BBa_K1033282 to DH5alpha. This was then grown in LB medium supplemented with chloramphenicol at 37 degrees Celsius. The first graph shows changes in OD588 over time, the second shows changes in OD588 / OD800 ratio with time, and the third shows OD588 according to OD800.