Difference between revisions of "Part:BBa K1061003"
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1061003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1061003 SequenceAndFeatures</partinfo>
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====Functional experiment====
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We test the death-induction effect of RIP 3 in two kinds of cell lines, here's the result.We also characterized it quantitively by using FACS.
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[[Image:Suicidegene-rip3-new.png|thumb|center|500px|functional experiment of RIP 3]]
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====kill range====
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For each suicide gene, it is important to know which cancer cells and normal cells it will kill.So that people can speficially choose gene that will be functional in their project when duelling about cancer. And we define this new feature for each suicide gene that we submitted. The list will be changed due to more and more experiment will be done. We highly recommend people to adopt this feature as an important characteristic of suicide gene.
  
  
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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==Functional Parameters: Austin_UTexas==
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<html>
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<body>
 
<partinfo>BBa_K1061003 parameters</partinfo>
 
<partinfo>BBa_K1061003 parameters</partinfo>
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<h3><center>Burden Imposed by this Part:</center></h3>
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<figure>
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<div class = "center">
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<center><img src = "https://static.igem.org/mediawiki/parts/f/fa/T--Austin_Utexas--no_burden_icon.png" style = "width:160px;height:120px"></center>
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</div>
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<figcaption><center><b>Burden Value: 2.5 ± 11.9% </b></center></figcaption>
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</figure>
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<p> Burden is the percent reduction in the growth rate of <i>E. coli</i> cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the
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<a href="https://parts.igem.org/Part:BBa_K3174002">BBa_K3174002</a> - <a href="https://parts.igem.org/Part:BBa_K3174007">BBa_K3174007</a> pages for more information on the methods, an explanation of the sources of burden,  and other conclusions from a large-scale measurement project conducted by the <a href="http://2019.igem.org/Team:Austin_UTexas">2019 Austin_UTexas team</a>.</p>
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<p>This functional parameter was added by the <a href="https://2020.igem.org/Team:Austin_UTexas/Contribution">2020 Austin_UTexas team.</a></p>
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</body>
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</html>
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<hr>
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<h2>References:</h2>
 
<h2>References:</h2>
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<p>[1]Liming Sun,Huayi Wang, et al,2012,Mixed Lineage Kinase Domain-like
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<p>[1] Duan-Wu Zhang et al. 2009,RIP3, an Energy Metabolism Regulator That Switches TNF-Induced Cell Death from Apoptosis to Necrosis,Science 325:332-336
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Protein Mediates Necrosis Signaling Downstream of RIP3 Kinase
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Cell,148:213-227
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Latest revision as of 18:44, 3 September 2020

RIP 3

Receptor interacting protein 3, also a serine/threonine kinase that play an important role in cell apoptosis and necrosis. It can induce necrosis via the interaction with RIP 1.[1]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 623
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 267
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1198

Functional experiment

We test the death-induction effect of RIP 3 in two kinds of cell lines, here's the result.We also characterized it quantitively by using FACS.

functional experiment of RIP 3

kill range

For each suicide gene, it is important to know which cancer cells and normal cells it will kill.So that people can speficially choose gene that will be functional in their project when duelling about cancer. And we define this new feature for each suicide gene that we submitted. The list will be changed due to more and more experiment will be done. We highly recommend people to adopt this feature as an important characteristic of suicide gene.



Functional Parameters: Austin_UTexas

BBa_K1061003 parameters

Burden Imposed by this Part:

Burden Value: 2.5 ± 11.9%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.



References:

[1] Duan-Wu Zhang et al. 2009,RIP3, an Energy Metabolism Regulator That Switches TNF-Induced Cell Death from Apoptosis to Necrosis,Science 325:332-336