Difference between revisions of "Part:BBa K1073024:Experience"

Latest revision as of 12:31, 28 September 2013

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Please enter how you used this part and how it worked out.

Applications of BBa_K1073024

When amilGFP is expressed, the colonies on plates as well as the liquid culture become yellow.

User Reviews

UNIQ3d3e4579c4d3e872-partinfo-00000000-QINU

•••••

iGEM Team Braunschweig 2013

When expressed the chromoprotein amilGFP exhibits a strong yellow color. In order to avoid absorption by the chomoproteins during the measurement of the OD of bacterial cultures, the absorption and emission spectra were measured and an optimal wavelength for spectral optical density measurement was determined to be at 520nm.

iGEM Team Braunschweig 2013: Absorption spectrum of chromoprotein amilGFP in the wavelength range 400-800 nm.

Absorption maximum of amilGFP: 504 nm

iGEM Team Braunschweig 2013: Emission spectrum of chromoprotein amilGFP. This chromoprotein does show significant emission that can be detected by different optical devices with the appropriate filters.

Emission maximum of amilGFP: 515,8 nm

Although this device is working as expected, it was not used by the iGEM Team Braunschweig 2013 since with the naked eye the yellow color is not as easy to distinguish from the media as the color of other chromoproteins like eforRed (BBa_K1073022) or aeBlue (BBa_K1073020).

iGEM Team Braunschweig 2013: Culture broth of E.coli XL1 Blue MRF' expressing different chromoproteins in monoculture and mixed.

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UNIQ3d3e4579c4d3e872-partinfo-00000004-QINU

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 ===User Reviews===
 <!-- DON'T DELETE --><partinfo>BBa_K1073024 StartReviews</partinfo>
-<!-- Template for a user review
 {|width='80%' style='border:1px solid gray'
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 |width='10%'|
-<partinfo>BBa_K1073024 AddReview number</partinfo>
+<partinfo>BBa_K1073024 AddReview 5</partinfo>
-<I>Username</I>
+<I>iGEM Team Braunschweig 2013</I>
 |width='60%' valign='top'|
-Enter the review inofrmation here.
+When expressed the chromoprotein amilGFP exhibits a strong yellow color. In order to avoid absorption by the chomoproteins during the measurement of the OD of bacterial cultures, the absorption and emission spectra were measured and an optimal wavelength for spectral optical density measurement was determined to be at 520nm.
+[[File:BSAbsorption-amilGFP.jpg |400px]]
+'''iGEM Team Braunschweig 2013:''' Absorption spectrum of chromoprotein amilGFP in the wavelength range 400-800 nm.
+'''Absorption maximum of amilGFP:'''  504 nm
+[[File:BS2013Emission amilGFP.jpg |400px]]
+'''iGEM Team Braunschweig 2013:''' Emission spectrum of chromoprotein amilGFP. This chromoprotein does show significant emission that can be detected by different optical devices with the appropriate filters.
+'''Emission maximum of amilGFP:''' 515,8 nm
+Although this device is working as expected, it was not used by the iGEM Team Braunschweig 2013 since with the naked eye the yellow color is not as easy to distinguish from the media as the color of other chromoproteins like eforRed (<partinfo>BBa_K1073022</partinfo>) or aeBlue (<partinfo>BBa_K1073020</partinfo>).
+<br>
+<br>
+[[File:Compraring chromoproteins.jpg |300px]]
+'''iGEM Team Braunschweig 2013:''' Culture broth of E.coli XL1 Blue MRF' expressing different chromoproteins in monoculture and mixed.
 |};
 <!-- End of the user review template -->
 <!-- DON'T DELETE --><partinfo>BBa_K1073024 EndReviews</partinfo>