Difference between revisions of "Part:BBa K1031913"
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| + | <p>For detailed information concerning XylS, please visit <a href="http://2013.igem.org/Team:Peking/Project/BioSensors/XylS">2013 Peking iGEM Biosensor XylS</a></p> | ||
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| − | In order to fine-tune the performance of XylS biosensor, we constructed a library of ''Pc'' constitutive promoters at different intensity. | + | In order to fine-tune the performance of XylS biosensor, we constructed a library of ''Pc'' constitutive promoters at different intensity. BBa_K1031913 is composed of three elements, the constitutive ''Pc'' promoter <html><a href="https://parts.igem.org/Part:BBa_J23109">J23109</a></html>, coding sequence of XylS and terminator <html><a href="https://parts.igem.org/Part:BBa_B0015">B0015</a></html>. ('''Fig 1''') |
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| − | <img src="https://static.igem.org/mediawiki/2013/thumb/1/11/Peking2013_part_109-XylS-TT.png/800px-Peking2013_part_109-XylS-TT.png" | + | <img src="https://static.igem.org/mediawiki/2013/thumb/1/11/Peking2013_part_109-XylS-TT.png/800px-Peking2013_part_109-XylS-TT.png" style="width:600px; margin-left:170px" /> |
| + | <p style="text-align:center"><b>Fig 1</b> Construction of biosensor circuit <i>Pm</i>/J23109-XylS. Orange arrowheads represent promoters. RBSs are shown as green ovals and squares in dark red stand for B0015 terminators. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K1031913 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1031913 SequenceAndFeatures</partinfo> | ||
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| − | Induction ratio of XylS biosensor library from ON/OFF test that utilizes different Pc promoters to control the expression of XylS. The dashed box refers to data for ''Pm''/J23109-XylS biosensor circuit. ('''Fig 2''') | + | Induction ratio of XylS biosensor library from ON/OFF test that utilizes different'' Pc'' promoters to control the expression of XylS. The dashed box refers to data for ''Pm''/J23109-XylS biosensor circuit. ('''Fig 2''') |
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| − | <img src="https://static.igem.org/mediawiki/2013/thumb/b/b9/Peking2013_part_Pc_library.png/800px-Peking2013_part_Pc_library.png" | + | <img src="https://static.igem.org/mediawiki/2013/thumb/b/b9/Peking2013_part_Pc_library.png/800px-Peking2013_part_Pc_library.png" style="width:600px; margin-left:170px" /> |
| + | <p style="text-align:center"><b>Fig 2</b> Horizontal axis stands for different XylS biosensor with <i>Pc</i> promoters of different strength. The expression strength of these constitutive promoters, J23113, J23109, J23114, J23105, J23106 is 21, 106, 256, 623, and 1185, respectively, according to the Parts'registry. Four kinds of aromatics, namely BzO, 2-MxeBzO, 3-MeBzO and 4-MeBzO, shown with different color intensities, were tested following <a href="http://2013.igem.org/Team:Peking/Team/Notebook/Protocols">Test Protocol 1</a>. Vertical axis represents the ON/OFF induction ratio. The dashed box refers to performance of <i>Pm</i>/J23109-XylS biosensor circuit. | ||
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Latest revision as of 23:17, 27 September 2013
J23109-XylS-Terminator
For detailed information concerning XylS, please visit 2013 Peking iGEM Biosensor XylS
Characterization
In order to fine-tune the performance of XylS biosensor, we constructed a library of Pc constitutive promoters at different intensity. BBa_K1031913 is composed of three elements, the constitutive Pc promoter J23109, coding sequence of XylS and terminator B0015. (Fig 1)
Fig 1 Construction of biosensor circuit Pm/J23109-XylS. Orange arrowheads represent promoters. RBSs are shown as green ovals and squares in dark red stand for B0015 terminators.
Sequence and Features
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 93
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 815
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 278
Data shown
Induction ratio of XylS biosensor library from ON/OFF test that utilizes different Pc promoters to control the expression of XylS. The dashed box refers to data for Pm/J23109-XylS biosensor circuit. (Fig 2)
Fig 2 Horizontal axis stands for different XylS biosensor with Pc promoters of different strength. The expression strength of these constitutive promoters, J23113, J23109, J23114, J23105, J23106 is 21, 106, 256, 623, and 1185, respectively, according to the Parts'registry. Four kinds of aromatics, namely BzO, 2-MxeBzO, 3-MeBzO and 4-MeBzO, shown with different color intensities, were tested following Test Protocol 1. Vertical axis represents the ON/OFF induction ratio. The dashed box refers to performance of Pm/J23109-XylS biosensor circuit.