Difference between revisions of "Part:BBa R0040:Design"

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__NOTOC__
 
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<partinfo>BBa_R0040 short</partinfo>
 
<partinfo>BBa_R0040 short</partinfo>
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===Design Notes===
 
===Design Notes===
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<partinfo>BBa_R0040</partinfo> (TetR repressible promoter) is based on a cI promoter. It has been modified to include two TetR binding sites.
  
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Constructed by DNA synthesis.
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BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified      to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
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===Source===
 
===Source===
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See <cite>Lutz-1997</cite>.
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Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210.
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===References===
 
===References===
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<biblio>
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* [http://nar.oxfordjournals.org/cgi/content/full/25/6/1203 Lutz R, Bujard H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10.]
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#Lutz-1997 pmid=9092630
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</biblio>

Latest revision as of 23:43, 7 March 2008

TetR repressible promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa_R0040 (TetR repressible promoter) is based on a cI promoter. It has been modified to include two TetR binding sites.

Constructed by DNA synthesis.

Source

See Lutz-1997.

References

<biblio>

  1. Lutz-1997 pmid=9092630

</biblio>