Difference between revisions of "Part:BBa K1073001:Experience"

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===Applications of BBa_K1073001===
 
===Applications of BBa_K1073001===
  
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The iGEM Team Braunschweig 2013 used this promoter in combination with an ampicillin resistance to induce growth by RhlR and N-buturyl-HSL in different ''E.coli'' strains. When inducing the regulated promoter a higher growth rate was observed.
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The iGEM Team Braunschweig 2013 used this device in combination with an ampicillin resistance gene (<partinfo>BBa_K1073011</partinfo>) to induce growth by RhlR and N-butyryl-HSL of different ''E.coli'' strains in ampicillin containing media.
  
 
===User Reviews===
 
===User Reviews===
 
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<partinfo>BBa_K1073001 AddReview number</partinfo>
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<partinfo>BBa_K1073001 AddReview 4 </partinfo>
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<I>Username</I>
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<I>iGEM Team Braunschweig 2013</I>
 
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Enter the review inofrmation here.
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Using this device in combination with an ampicillin resistance gene cells showed a higher growth rate in ampicillin containing media when the promoter was induced.
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The device shows leakiness to a constant low degree in yeast extract complex media. Background activity of the genes downstream of the promoter were detected in all experiments. However activity was much higher when the promoter was induced by RhlR and N-butyryl-HSL.  
 
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Latest revision as of 08:52, 17 October 2013


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1073001

The iGEM Team Braunschweig 2013 used this device in combination with an ampicillin resistance gene (BBa_K1073011) to induce growth by RhlR and N-butyryl-HSL of different E.coli strains in ampicillin containing media.

User Reviews

UNIQb54b39674f6b7891-partinfo-00000001-QINU

••••

iGEM Team Braunschweig 2013

Using this device in combination with an ampicillin resistance gene cells showed a higher growth rate in ampicillin containing media when the promoter was induced. The device shows leakiness to a constant low degree in yeast extract complex media. Background activity of the genes downstream of the promoter were detected in all experiments. However activity was much higher when the promoter was induced by RhlR and N-butyryl-HSL.

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UNIQb54b39674f6b7891-partinfo-00000003-QINU