Difference between revisions of "Part:BBa J45993:Design"

(→‎Source)
 
(5 intermediate revisions by 2 users not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J45993 short</partinfo>
 
<partinfo>BBa_J45993 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
Truncated Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GGCT TAT GTT TTC GCT GAT ATC - 3'
+
*Constructed by primer annealing and extension
  
−
Total Length: 49 bp
+
====Template====
 +
None.
  
−
Annealing Length: 21 bp
+
====Forward Primer====
 +
<code>5'- CTA GAG GCT TAT GTT TTC GCT GAT ATC CCG AGC GGT TTC AAA ATT GTG ATC TAT ATT TAA CAA ATA CTA GTA GCG GCC GCT GCA -3'</code>
  
−
GC Content: 38.1%
+
====Reverse Primer====
 +
<code>5'- GCG GCC GCT ACT AGT ATT TGT TAA ATA TAG ATC ACA ATT TTG AAA CCG CTC GGG ATA TCA GCG AAA ACA TAA GCC T -3'</code>
  
−
Melting Temperature: 49.5 degrees C
+
===Source===
 +
*Promoter controlling expression of ''osmY'' from ''Escherichia coli'' (GenBank [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=453110 S67278])
  
−
hairpin deltaG: -2.66 kcal/mol
+
===References===
 +
<biblio>
 +
#Yim-1994 pmid=8282684
 +
#Schellhorn-1998 pmid=9829938
 +
#Wise pmid=8631665
 +
#Funabashi pmid=11879714
 +
#Makinoshima pmid=11985708
 +
#Yim-1992 pmid=1317380
 +
#Hengge-Aronis-1993 pmid=8416901
 +
#Vijaykumar-2004 pmid=15576800
 +
</biblio>
  
−
self dimer deltaG: -99.97 kcal/mol
 
  
−
Reverse Primer: 5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG- 3'
+
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=453110 S67278]
  
−
Total Length: 54 bp
+
Neidhart, Frederick C. ''Escherichia coli and Salmonella''. Washington: ASM Press, 1996.
−
 
+
−
Annealing Length: 25 bp
+
−
 
+
−
GC Content: 20.0%
+
−
 
+
−
Melting Temperature: 46.4 degrees C
+
−
 
+
−
hairpin deltaG: -2.86 kcal/mol
+
−
 
+
−
self dimer deltaG: -99.44 kcal/mol
+
−
 
+
−
heterodimer deltaG with Conserved Forward Primer: -99.97 kcal/mol
+
−
 
+
−
 
+
−
 
+
−
===Source===
+
−
 
+
−
This part was PCRed out of E. coli's genome with the primers listed in the Design Notes section.
+
−
 
+
−
===References===
+

Latest revision as of 23:38, 7 March 2008

Minimal stationary phase osmY promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • Constructed by primer annealing and extension

Template

None.

Forward Primer

5'- CTA GAG GCT TAT GTT TTC GCT GAT ATC CCG AGC GGT TTC AAA ATT GTG ATC TAT ATT TAA CAA ATA CTA GTA GCG GCC GCT GCA -3'

Reverse Primer

5'- GCG GCC GCT ACT AGT ATT TGT TAA ATA TAG ATC ACA ATT TTG AAA CCG CTC GGG ATA TCA GCG AAA ACA TAA GCC T -3'

Source

  • Promoter controlling expression of osmY from Escherichia coli (GenBank [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=453110 S67278])

References

<biblio>

  1. Yim-1994 pmid=8282684
  2. Schellhorn-1998 pmid=9829938
  3. Wise pmid=8631665
  4. Funabashi pmid=11879714
  5. Makinoshima pmid=11985708
  6. Yim-1992 pmid=1317380
  7. Hengge-Aronis-1993 pmid=8416901
  8. Vijaykumar-2004 pmid=15576800

</biblio>


[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=453110 S67278]

Neidhart, Frederick C. Escherichia coli and Salmonella. Washington: ASM Press, 1996.