Difference between revisions of "Part:BBa K1078001"
 
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<partinfo>BBa_K1078001 short</partinfo>
 
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This device is composed by three parts. The first part is a modified promoter pAOX1, it was found in a library where it showed more than 60% stronger activation when compared with the wild-type, as reported by Hartner FS et al., in Promoter library designed for fine-tuned gene expression in Pichia pastoris. Nucleic Acids Res 2008, 36:e76. The second part is a kozak sequence for Pichia pastoris, and the third is a Red fluorescent protein condon optimized for expression in Pichia pastoris.
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This device was created to express a reporter gene, in this case RFP, in the presence of ethanol, its expression is inactivated by ethanol. When combined with the modified Mxr1 (BBa_K1078000), it is not inactivated by ethanol. This is useful for our biosensor design, which aims to detect levels of methanol above 2% in common alcoholic drinks (normally containing 10 to 60% ethanol). This will allow the government to make a preliminary high-throughput screening of ethanol drinks tainted will methanol.
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<p>This device is composed of three parts. The first part is a modified promoter P<sub>AOX1</sub>; it was found in a library where it showed more than 60% stronger activation when compared with the wild-type P<sub>AOX1</sub> (found as <a href="https://parts.igem.org/Part:BBa_K431007">BBa_K431007</a> in the registry of parts), as reported by Hartner FS et al., in <i>Promoter library designed for fine-tuned gene expression inPichia pastoris. Nucleic Acids Res 2008, 36:e76</i>. The second part is a kozak sequence for <i>P. pastoris</i>, and the third is a red fluorescent protein condon optimized for expression in <i>P. pastoris</i>.</p>
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<p>This device was created to express a reporter gene, in this case RFP, in the presence of methanol. Its expression is inactivated by ethanol. When combined with the modified Mxr1 (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1078000">BBa_K1078000</a>), it is not inactivated by ethanol. This is useful for our biosensor design, which aims to detect levels of methanol above 2% in common alcoholic drinks (normally containing 10 to 60% ethanol). This will allow government to make a preliminary high-throughput screening of ethanol drinks tainted will methanol. </p>
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<!-- Add more about the biology of this part here
 
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Latest revision as of 18:28, 27 September 2013

Strong reporter device optimized for Pichia pastoris, activated by methanol

This device is composed of three parts. The first part is a modified promoter PAOX1; it was found in a library where it showed more than 60% stronger activation when compared with the wild-type PAOX1 (found as BBa_K431007 in the registry of parts), as reported by Hartner FS et al., in Promoter library designed for fine-tuned gene expression inPichia pastoris. Nucleic Acids Res 2008, 36:e76. The second part is a kozak sequence for P. pastoris, and the third is a red fluorescent protein condon optimized for expression in P. pastoris.

This device was created to express a reporter gene, in this case RFP, in the presence of methanol. Its expression is inactivated by ethanol. When combined with the modified Mxr1 (BBa_K1078000), it is not inactivated by ethanol. This is useful for our biosensor design, which aims to detect levels of methanol above 2% in common alcoholic drinks (normally containing 10 to 60% ethanol). This will allow government to make a preliminary high-throughput screening of ethanol drinks tainted will methanol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]