Difference between revisions of "Part:BBa K844017"

Latest revision as of 19:30, 25 October 2012

Spider Silk Generator - 10x "F" Silk Construct with His Tag

Protein generator part for Spider Silk. Protein is a 10x fusion of spider silk "F" subunits (1x BBa_K844007 followed by 9x BBa_K844003), which were codon optimized to use a minimal set of tRNA codons (1 codon per amino acid). Contains a lactose/IPTG inducible promoter, RBS, and a 10x-His tag (BBa_K844000) for purification of protein.

IMPORTANT NOTE: This part uses Assembly Standard #23 (Silver Fusion) scar sites, which the sequence data below and on composite parts using this part will not reflect this scar sequence (it shows Assembly Standard #10 scars).

Related Parts

BBa_K844000 - A 10x-His tag with double stop codons (TAATAA) which was vital to the creation of the gel and blot shown above.

BBa_K844002 - The non-optimized native spider silk subunit that K8440016 is a codon optimized and improved concatemer of.

BBa_K844016 - Another silk generator, this part uses the "B" subunit and has only 4x repeat units.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K844017 short</partinfo>
-Description Goes Here
+Protein generator part for Spider Silk. Protein is a 10x fusion of spider silk "F" subunits (1x [https://parts.igem.org/Part:BBa_K844007 BBa_K844007] followed by 9x [https://parts.igem.org/Part:BBa_K844003 BBa_K844003]), which were codon optimized to use a minimal set of tRNA codons (1 codon per amino acid). Contains a lactose/IPTG inducible promoter, RBS, and a 10x-His tag ([https://parts.igem.org/Part:BBa_K844000 BBa_K844000]) for purification of protein.
+IMPORTANT NOTE: This part uses [https://parts.igem.org/Assembly_standard_23 Assembly Standard #23 (Silver Fusion)] scar sites, which the sequence data below and on composite parts using this part will not reflect this scar sequence (it shows [https://parts.igem.org/Assembly_standard_10 Assembly Standard #10] scars).
+===Related Parts===
+[https://parts.igem.org/Part:BBa_K844000 BBa_K844000] - A 10x-His tag with double stop codons (TAATAA) which was vital to the creation of the gel and blot shown above.
+[https://parts.igem.org/Part:BBa_K844002 BBa_K844002] - The non-optimized native spider silk subunit that K8440016 is a codon optimized and improved concatemer of.
-<!-- Add more about the biology of this part here
+[https://parts.igem.org/Part:BBa_K844016 BBa_K844016] - Another silk generator, this part uses the "B" subunit and has only 4x repeat units.
-===Usage and Biology===
 <!-- -->