Difference between revisions of "Part:BBa K801065:Design"

Latest revision as of 11:29, 16 October 2012

(+)-limonene synthase 1 with Strep-Tag

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1676
Illegal SapI.rc site found at 100

C-Terminal Strep-Tag added

See BBa_K801061

Keywords: limonen, strep-tag

Abbreviations:

Design Notes

Related BioBrick:

Quality control measures:

Backbone:

Backbone name: pSB1C3
Resistance: Cp
Copynumber: high

Protein coding:

BioBrick encodes (+)-limonene synthase
UniProt entry: [http://www.uniprot.org/uniprot/Q8L5K3 Q8L5K3]

Enzymatic activity:

E.C. Number: [http://enzyme.expasy.org/EC/4.2.3.20 4.2.3.20]

Cytotoxicity:
cytotoxic for S. cervisiae growth inhibition at 1 mM, lethal effect at 100 mM

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:

BioBrick: BBa_I742111

Organism:

Gene sequence derived from Citrus limon

References

Literature references:

[http://www.ncbi.nlm.nih.gov/pubmed/12084056 Pubmed: Lücker, J., El Tamer, M. K., Schwab, W., Verstappen, F. W. A., van der Plas, L. H. W., Bouwmeester, H. J., and Verhoeven, H. A., 2002: Monoterpene biosynthesis in lemon (Citrus limon). cDNA isolation and functional analysis of four monoterpene synthases. Eur J Biochem, 269(13):3160–71]

Database references:

[http://www.ncbi.nlm.nih.gov/nucleotide/21435702 GenBank: Citrus limon (+)-limonene synthase 1 mRNA, complete cds]
[http://www.uniprot.org/uniprot/Q8L5K3 Uniprot: (R)-limonene synthase 1]

@@ Line 54: / Line 54: @@
 * E.C. Number: [http://enzyme.expasy.org/EC/4.2.3.20 4.2.3.20]
 '''Cytotoxicity:'''<br>
-<!--none/not known/cytotoxic for ''organism name''-->
+cytotoxic for ''S. cervisiae'' growth inhibition at 1 mM, lethal effect at 100 mM
 '''Safety notes:'''<br>
@@ Line 91: / Line 91: @@
 '''Literature references:'''<br>
-*[[http://www.ncbi.nlm.nih.gov/pubmed/12084056 Lücker et al., 2002]] Lücker, J., El Tamer, M. K., Schwab, W., Verstappen, F. W. A., van der Plas, L. H. W., Bouwmeester, H. J., and Verhoeven, H. A. (2002). Monoterpene biosynthesis in lemon (''Citrus limon''). cDNA isolation and functional analysis of four monoterpene synthases. ''Eur J Biochem'', 269(13):3160–71.
+*[http://www.ncbi.nlm.nih.gov/pubmed/12084056 '''Pubmed:''' Lücker, J., El Tamer, M. K., Schwab, W., Verstappen, F. W. A., van der Plas, L. H. W., Bouwmeester, H. J., and Verhoeven, H. A., 2002: Monoterpene biosynthesis in lemon (''Citrus limon''). cDNA isolation and functional analysis of four monoterpene synthases. ''Eur J Biochem'', 269(13):3160–71]
-<!--*[http://www.ncbi.nlm.nih.gov/pubmed/?PMID? '''Pubmed:''' ?Author(s)?, ?year?: ?title?]-->
 '''Database references:'''<br>
-<!--*[http://www.ncbi.nlm.nih.gov/nuccore/?accessNr? '''GenBank''': ?title?]-->
+*[http://www.ncbi.nlm.nih.gov/nucleotide/21435702 '''GenBank''': Citrus limon (+)-limonene synthase 1 mRNA, complete cds]
 <!--*[http://www.ebi.ac.uk/interpro/IEntry?ac=?accessNr? '''Interpro''': ?title?]-->
-<!--*[http://www.uniprot.org/uniprot/?accessNr? '''Uniprot''': ?title?]-->
+*[http://www.uniprot.org/uniprot/Q8L5K3 '''Uniprot''': (R)-limonene synthase 1]
 <!--*[http://pfam.sanger.ac.uk/family/?accessNr? '''Pfam:''' ?title?]-->
 <!--*[http://www.rcsb.org/pdb/explore/explore.do?structureId=?accessNR? '''PDB:''' ?tile?]-->
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