Difference between revisions of "Part:BBa J45005:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J45005 short</partinfo>
 
<partinfo>BBa_J45005 short</partinfo>
  
 
<partinfo>BBa_J45005 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_J45005 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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we need an MTA or to sequence the coding region for this enzyme
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*No BioBrick restriction enzyme cut sites in coding region
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*Changed natural stop codon to TAA TAA
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*No template DNA available and thus could not construct by PCR
  
 
===Source===
 
===Source===
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''SAMT'' from ''Clarkia breweri'' ([http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=6002711 GenBank AF133053])
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Genbank: AF133053, Eran Pichersky
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===References===
 
===References===
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<biblio>
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#Ross-ArchBiochemBiophys-1999 pmid=10375393
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</biblio>
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*[http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-6-6 MIT iGEM 2006 notebook entry]

Latest revision as of 22:01, 9 March 2008

SAM:salicylic acid carboxyl methyltransferase; converts salicylic acid to methyl salicylate (winterg


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 652
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 232

Design Notes

  • No BioBrick restriction enzyme cut sites in coding region
  • Changed natural stop codon to TAA TAA
  • No template DNA available and thus could not construct by PCR

Source

SAMT from Clarkia breweri ([http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=6002711 GenBank AF133053])

References

<biblio>

  1. Ross-ArchBiochemBiophys-1999 pmid=10375393

</biblio>

  • [http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-6-6 MIT iGEM 2006 notebook entry]