Difference between revisions of "Part:BBa K863122"

Latest revision as of 22:03, 26 September 2012

BBa_I13522 with His-tag

GFP with constant promoter and His-tag made from BBa_I13522 (without the terminators). Originally designed to have a comparison to a His-tagged GFP to cellulose binding domain fusion protein.

Usage and Biology

GFP (mut3b) [note that this part does not have a barcode]

Tagged version of gfp from Repressilator reporter. See the design page for more source information.

Fluorescence wavelengths

Cormack et al.Cormack report the following excitation and emission data for GFPmut3 -

Excitation max - 501nm
Emission max - 511nm

Latency

Cormack et al.Cormack report detectable fluorescence within 8 mins.

Pictures

BBa_I13522 visualized under non-UV lightbox
BBa_I13522 visualized under 254nm wavelength UV lightbox

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 813
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 724

@@ Line 3: / Line 3: @@
 GFP with constant promoter and His-tag made from [https://parts.igem.org/Part:BBa_I13522 BBa_I13522] (without the terminators).
-<!-- Add more about the biology of this part here
+Originally designed to have a comparison to a His-tagged GFP to cellulose binding domain fusion protein.
 ===Usage and Biology===
@@ Line 26: / Line 27: @@
 ====Latency====
 Cormack ''et al''.<cite>Cormack</cite> report detectable fluorescence within 8 mins.
-''Please add maturation time data for E0040 here.''
 ==Pictures==
 <gallery>